Analytical Data
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Gene name
DYRK1B
- Application
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Alternative Names
DYRK1B. MIRK
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9Y463
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Expression Region
1-629aa
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AA Sequence
MAVPPGHGPFSGFPGPQEHTQVLPDVRLLPRRLPLAFRDATSAPLRKLSVDLIKTYKHINEVYYAKKKRRAQQAPPQDSSNKKEKKVLNHGYDDDNHDYIVRSGERWLERYEIDSLIGKGSFGQVVKAYDHQTQELVAIKIIKNKKAFLNQAQIELRLLELMNQHDTEMKYYIVHLKRHFMFRNHLCLVFELLSYNLYDLLRNTHFRGVSLNLTRKLAQQLCTALLFLATPELSIIHCDLKPENILLCNPKRSAIKIVDFGSSCQLGQRIYQYIQSRFYRSPEVLLGTPYDLAIDMWSLGCILVEMHTGEPLFSGSNEVDQMNRIVEVLGIPPAAMLDQAPKARKYFERLPGGGWTLRRTKELRKDYQGPGTRRLQEVLGVQTGGPGGRRAGEPGHSPADYLRFQDLVLRMLEYEPAARISPLGALQHGFFRRTADEATNTGPAGSSASTSPAPLDTCPSSSTASSISSSGGSSGSSSDNRTYRYSNRYCGGPGPPITDCEMNSPQVPPSQPLRPWAGGDVPHKTHQAPASASSLPGTGAQLPPQPRYLGRPPSPTSPPPPELMDVSLVGGPADCSPPHPAPAPQHPAASALRTRMTGGRPPLPPPDDPATLGPHLGLRGVPQSTAASS
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Molecular Weight
95.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
DYRK1B (Dual-specificity tyrosine-phosphorylation-regulated kinase 1B) is a serine/threonine kinase that belongs to the DYRK family, characterized by its dual specificity for both serine/threonine and tyrosine residues. Research on DYRK1B has garnered significant attention due to its pivotal roles in various cellular processes, including cell proliferation, differentiation, and apoptosis. Dysregulation of DYRK1B has been implicated in several pathological conditions, including cancer and neurodegenerative diseases, making it a potential therapeutic target. Studies have shown that DYRK1B phosphorylation can influence signaling pathways, thereby affecting cellular responses to growth factors and stress. The exploration of DYRK1B as a biomarker and drug target necessitates the generation of recombinant DYRK1B protein for functional assays and structural studies. This recombinant protein is instrumental in elucidating its enzymatic activity, substrate specificity, and interaction with other signaling molecules. Understanding DYRK1B's mechanisms at the molecular level may provide insights into its involvement in disease progression and offer a foundation for developing novel therapeutic strategies. The recent advancements in protein expression systems and purification techniques have facilitated the production of high-quality DYRK1B recombinant protein, making it available for further biochemical and biophysical analyses essential for the discovery of DYRK1B inhibitors or modulators. Overall, the study of DYRK1B recombinant protein is crucial for advancing our knowledge of its biological significance and therapeutic potential.
