Analytical Data
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Gene name
FOXO1
- Application
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Alternative Names
FOXO1；FKHR；FOXO1A；Forkhead box Protein O1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q12778
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Expression Region
1-655aa
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AA Sequence
MAEAPQVVEI DPDFEPLPRP RSCTWPLPRP EFSQSNSATS SPAPSGSAAA NPDAAAGLPS ASAAAVSADF MSNLSLLEES EDFPQAPGSV AAAVAAAAAA AATGGLCGDF QGPEAGCLHP APPQPPPPGP LSQHPPVPPA AAGPLAGQPR KSSSSRRNAW GNLSYADLIT KAIESSAEKR LTLSQIYEWM VKSVPYFKDK GDSNSSAGWK NSIRHNLSLH SKFIRVQNEG TGKSSWWMLN PEGGKSGKSP RRRAASMDNN SKFAKSRSRA AKKKASLQSG QEGAGDSPGS QFSKWPASPG SHSNDDFDNW STFRPRTSSN ASTISGRLSP IMTEQDDLGE GDVHSMVYPP SAAKMASTLP SLSEISNPEN MENLLDNLNL LSSPTSLTVS TQSSPGTMMQ QTPCYSFAPP NTSLNSPSPN YQKYTYGQSS MSPLPQMPIQ TLQDNKSSYG GMSQYNCAPG LLKELLTSDS PPHNDIMTPV DPGVAQPNSR VLGQNVMMGP NSVMSTYGSQ ASHNKMMNPS SHTHPGHAQQ TSAVNGRPLP HTVSTMPHTS GMNRLTQVKT PVQVPLPHPM QMSALGGYSS VSSCNGYGRM GLLHQEKLPS DLDGMFIERL DCDMESIIRN DLMDGDTLDF NFDNVLPNQS FPHSVKTTTH SWVSG
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
FOXO1, a member of the Forkhead box O (FOXO) transcription factor family, plays a pivotal role in various biological processes, including metabolism, cell cycle regulation, and apoptosis. Its significance in insulin signaling and stress resistance has made it a focal point in diabetes and cancer research. Dysregulation of FOXO1 has been implicated in the pathogenesis of numerous diseases, prompting investigations into its functional roles and regulatory mechanisms. Researchers have explored FOXO1's ability to modulate gene expression in response to oxidative stress and nutrient availability, revealing its influence on cellular homeostasis and longevity. The study of FOXO1 recombinant proteins has emerged as a critical approach to elucidate the specific pathways and interactions in which FOXO1 is involved, particularly in manipulating its activity for therapeutic benefits. By generating recombinant FOXO1 proteins, scientists can investigate the structural and functional dynamics of FOXO1 in various cellular contexts, providing insights into its regulatory mechanisms and potential as a drug target. Furthermore, understanding how FOXO1 interacts with other signaling pathways can lead to novel strategies for overcoming insulin resistance and inhibiting tumor growth, marking FOXO1 as a promising candidate for future biomedical applications. Overall, the research on FOXO1 recombinant proteins is crucial for advancing our knowledge of this transcription factor and developing targeted therapies for metabolic disorders and cancer, reflecting its importance in maintaining cellular health and function.
