Analytical Data
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Gene name
VPS45
- Application
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Alternative Names
h-VPS45; H1; H1VPS45; HGNC:14579; hlVps45; Leucocyte vacuolar Protein sorting 45; OTTHUMP00000014482; Vacuolar Protein sorting 45 homolog (S. cerevisiae); Vacuolar Protein sorting 45A (yeast homolog); Vacuolar Protein sorting 45A; Vacuolar Protein sorting 45B (yeast); Vacuolar Protein sorting-associated Protein 45; Vps45; VPS45_HUMAN; VPS45A; VPS45B; VPS54A; VSP45; VSP45A
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NRW7
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Expression Region
1-570 aa
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AA Sequence
MNVVFAVKQY ISKMIEDSGP GMKVLLMDKE TTGIVSMVYT QSEILQKEVY LFERIDSQNR EIMKHLKAIC FLRPTKENVD YIIQELRRPK YTIYFIYFSN VISKSDVKSL AEADEQEVVA EVQEFYGDYI AVNPHLFSLN ILGCCQGRNW DPAQLSRTTQ GLTALLLSLK KCPMIRYQLS SEAAKRLAEC VKQVITKEYE LFEFRRTEVP PLLLILDRCD DAITPLLNQW TYQAMVHELL GINNNRIDLS RVPGISKDLR EVVLSAENDE FYANNMYLNF AEIGSNIKNL MEDFQKKKPK EQQKLESIAD MKAFVENYPQ FKKMSGTVSK HVTVVGELSR LVSERNLLEV SEVEQELACQ NDHSSALQNI KRLLQNPKVT EFDAARLVML YALHYERHSS NSLPGLMMDL RNKGVSEKYR KLVSAVVEYG GKRVRGSDLF SPKDAVAITK QFLKGLKGVE NVYTQHQPFL HETLDHLIKG RLKENLYPYL GPSTLRDRPQ DIIVFVIGGA TYEEALTVYN LNRTTPGVRI VLGGTTVHNT KSFLEEVLAS GLHSRSKESS QVTSRSASRR
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Molecular Weight
65 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
VPS45 is a protein that plays a crucial role in vesicular transport within cells, particularly in the secretory and endocytic pathways. It is part of the Sec1/Munc18 (SM) protein family, which is known to facilitate the fusion of transport vesicles with their target membranes by interacting with soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs). The study of VPS45 and its functional mechanisms is important due to its implications in various cellular processes, including neurotransmitter release, hormone secretion, and recycling of membrane proteins. Dysregulation of VPS45 function has been linked to several diseases, including neurodegenerative disorders and metabolic syndromes. Research has focused on understanding the structural basis of VPS45 interactions with SNARE proteins and other partners, as well as the regulatory mechanisms governing its activity. Additionally, scientists are investigating how mutations or alterations in VPS45 expression can disrupt intracellular transport, leading to pathological conditions. By elucidating the role of VPS45 in cellular dynamics, researchers aim to develop targeted therapeutic strategies that can restore normal function and mitigate disease symptoms associated with VPS45 dysfunction. This area of study is particularly relevant in the context of improving our understanding of cellular communication and transport mechanisms, which are essential for maintaining cellular homeostasis.
