Analytical Data
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Gene name
SPC25
- Application
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Alternative Names
Kinetochore protein Spc25. hSpc25
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9HBM1
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Expression Region
1-224 aa
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AA Sequence
MVEDELALFDKSINEFWNKFKSTDTSCQMAGLRDTYKDSIKAFAEKLSVKLKEEERMVEMFLEYQNQISRQNKLIQEKKDNLLKLIAEVKGKKQELEVLTANIQDLKEEYSRKKETISTANKANAERLKRLQKSADLYKDRLGLEIRKIYGEKLQFIFTNIDPKNPESPFMFSLHLNEARDYEVSDSAPHLEGLAEFQENVRKTNNFSAFLANVRKAFTATVYN
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Molecular Weight
52.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
SPC25 is a crucial component of the spindle pole body that plays a significant role in the mitotic process, particularly during the formation and function of the spindle apparatus in cell division. Research on SPC25 recombinant proteins has gained momentum due to their potential implications in understanding cell cycle regulation and the mechanics of mitosis, which are fundamental to various physiological processes and diseases, especially cancer. The study of SPC25 proteins enables scientists to explore their interactions with other mitotic proteins and their participation in the assembly and stability of the spindle apparatus. With advancements in techniques for protein engineering and expression systems, researchers can now produce high yields of recombinant SPC25, facilitating detailed biochemical and structural analyses. This research not only enhances our understanding of the mitotic machinery but also paves the way for developing targeted therapeutic strategies that could disrupt aberrant cell division in cancer cells. Ultimately, the investigation of SPC25 and its functional dynamics serves as a pivotal avenue in cell biology, honing in on the intricate regulatory networks that govern cellular proliferation and their potential breakdown in oncogenic processes.
