Analytical Data
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Gene name
SPAG4
- Application
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Alternative Names
SPAG4; SUN4; Sperm-associated antigen 4 protein; Outer dense fiber-associated protein SPAG4; SUN domain-containing protein 4
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NPE6
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Expression Region
1-379 aa
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AA Sequence
MPRSSRSPGDPGALLEDVAHNPRPRRIAQRGRNTSRMAEDTSPNMNDNILLPVRNNDQALGLTQCMLGCVSWFTCFACSLRTQAQQVLFNTCRCKLLCQKLMEKTGILLLCAFGFWMFSIHLPSKMKVWQDDSINGPLQSLRLYQEKVRHHSGEIQDLRGSMNQLIAKLQEMEAMSDEQKMAQKIMKMIHGDYIEKPDFALKSIGASIDFEHTSVTYNHEKAHPYWNWIQLWNYAQPPDVILEPNVTPGNCWAFEGDRGQVTIQLAQKVYLSNLTLQHIPKTISLSGSLDTAPKDFVIYGMEGSPKEEVFLGAFQFQPENIIQMFPLQNQPARAFSAVKVKISSNWGNPGFTCLYRVRVHGSVAPPREQPHQNPYPKRD
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Molecular Weight
67.21 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SPAG4 (Sperm Associated Antigen 4) is a protein primarily expressed in the male reproductive system, particularly in spermatogenic cells and mature sperm. Its role in spermatogenesis and sperm function has garnered interest due to its potential implications in male fertility. Research has shown that SPAG4 is involved in sperm motility and may play a crucial role in the interaction between sperm and the egg during fertilization. Notably, mutations or dysregulation of the SPAG4 gene have been associated with male infertility, making it a candidate for investigating infertility mechanisms and potential therapeutic targets. Additionally, SPAG4 is being explored for its relevance in various cancers, given its expression in some tumor types. The study of SPAG4 recombinant proteins has opened avenues for better understanding its biological functions, functional assays, and the development of diagnostic tools for male infertility. Researchers are focusing on purifying and characterizing SPAG4 to elucidate its structure-function relationships and to explore its potential as a biomarker for reproductive health assessment. The exploration of SPAG4's role extends beyond reproductive biology, as it may also hold significance in cell signaling processes and tissue development, leading to a broader understanding of its implications in health and disease. The ongoing research into SPAG4 and its recombinant forms is paving the way for advances in reproductive medicine and potential interventions for infertility.
