Analytical Data
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基因名
GRK4
- Application
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别名
GRK4；GPRK2L；GPRK4；G Protein-coupled receptor kinase 4
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P32298
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表达区间
140-578aa
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氨基酸序列
KAFEECTRVAHNYLRGEPFEEYQESSYFSQFLQWKWLERQPVTKNTFRHY RVLGKGGFGEVCACQVRATGKMYACKKLQKKRIKKRKGEAMALNEKRILE KVQSRFVVSLAYAYETKDALCLVLTIMNGGDLKFHIYNLGNPGFDEQRAV FYAAELCCGLEDLQRERIVYRDLKPENILLDDRGHIRISDLGLATEIPEG QRVRGRVGTVGYMAPEVVNNEKYTFSPDWWGLGCLIYEMIQGHSPFKKYK EKVKWEEVDQRIKNDTEEYSEKFSEDAKSICRMLLTKNPSKRLGCRGEGA AGVKQHPVFKDINFRRLEANMLEPPFCPDPHAVYCKDVLDIEQFSVVKGI YLDTADEDFYARFATGCVSIPWQNEMIESGCFKDINKSESEEALPLDLDK NIHTPVSRPNRGFFYRLFRRGGCLTMVPSEKEVEPKQC
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GRK4 (G-protein coupled receptor kinase 4) is a key enzyme that plays a critical role in regulating G-protein coupled receptor (GPCR) signaling, which is essential for various physiological processes. Dysregulation of GRK4 has been implicated in several cardiovascular and renal diseases, including hypertension and heart failure. Research into GRK4 has revealed that genetic polymorphisms in the GRK4 gene can affect its activity and expression, contributing to individual differences in disease susceptibility and drug response. The recombinant expression of GRK4 protein in suitable expression systems allows for in-depth studies on its structure, function, and regulatory mechanisms. By utilizing methods such as bacterial or eukaryotic expression systems, researchers can produce large quantities of GRK4 for biochemical, biophysical, and structural analyses. Understanding the molecular dynamics of GRK4 can provide insights into its role in GPCR desensitization and internalization, paving the way for the development of targeted therapies that can modulate its activity. Given the increasing recognition of GRK4 as a potential therapeutic target, ongoing research aims to delineate its precise functions and interactions, ultimately contributing to novel strategies for treating diseases associated with GPCR dysregulation.
