Analytical Data
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Gene name
GRK4
- Application
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Alternative Names
GRK4；GPRK2L；GPRK4；G Protein-coupled receptor kinase 4
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P32298
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Expression Region
140-578aa
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AA Sequence
KAFEECTRVAHNYLRGEPFEEYQESSYFSQFLQWKWLERQPVTKNTFRHY RVLGKGGFGEVCACQVRATGKMYACKKLQKKRIKKRKGEAMALNEKRILE KVQSRFVVSLAYAYETKDALCLVLTIMNGGDLKFHIYNLGNPGFDEQRAV FYAAELCCGLEDLQRERIVYRDLKPENILLDDRGHIRISDLGLATEIPEG QRVRGRVGTVGYMAPEVVNNEKYTFSPDWWGLGCLIYEMIQGHSPFKKYK EKVKWEEVDQRIKNDTEEYSEKFSEDAKSICRMLLTKNPSKRLGCRGEGA AGVKQHPVFKDINFRRLEANMLEPPFCPDPHAVYCKDVLDIEQFSVVKGI YLDTADEDFYARFATGCVSIPWQNEMIESGCFKDINKSESEEALPLDLDK NIHTPVSRPNRGFFYRLFRRGGCLTMVPSEKEVEPKQC
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GRK4 (G-protein coupled receptor kinase 4) is a key enzyme that plays a critical role in regulating G-protein coupled receptor (GPCR) signaling, which is essential for various physiological processes. Dysregulation of GRK4 has been implicated in several cardiovascular and renal diseases, including hypertension and heart failure. Research into GRK4 has revealed that genetic polymorphisms in the GRK4 gene can affect its activity and expression, contributing to individual differences in disease susceptibility and drug response. The recombinant expression of GRK4 protein in suitable expression systems allows for in-depth studies on its structure, function, and regulatory mechanisms. By utilizing methods such as bacterial or eukaryotic expression systems, researchers can produce large quantities of GRK4 for biochemical, biophysical, and structural analyses. Understanding the molecular dynamics of GRK4 can provide insights into its role in GPCR desensitization and internalization, paving the way for the development of targeted therapies that can modulate its activity. Given the increasing recognition of GRK4 as a potential therapeutic target, ongoing research aims to delineate its precise functions and interactions, ultimately contributing to novel strategies for treating diseases associated with GPCR dysregulation.
