Analytical Data
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Gene name
DDX6
- Application
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Alternative Names
DDX6; HLR2; RCK; Probable ATP-dependent RNA helicase DDX6; EC 3.6.4.13; ATP-dependent RNA helicase p54; DEAD box protein 6; Oncogene RCK
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P26196
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Expression Region
1-483aa
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AA Sequence
MSTARTENPVIMGLSSQNGQLRGPVKPTGGPGGGGTQTQQQMNQLKNTNTINNGTQQQAQSMTTTIKPGDDWKKTLKLPPKDLRIKTSDVTSTKGNEFEDYCLKRELLMGIFEMGWEKPSPIQEESIPIALSGRDILARAKNGTGKSGAYLIPLLERLDLKKDNIQAMVIVPTRELALQVSQICIQVSKHMGGAKVMATTGGTNLRDDIMRLDDTVHVVIATPGRILDLIKKGVAKVDHVQMIVLDEADKLLSQDFVQIMEDIILTLPKNRQILLYSATFPLSVQKFMNSHLQKPYEINLMEELTLKGVTQYYAYVTERQKVHCLNTLFSRLQINQSIIFCNSSQRVELLAKKISQLGYSCFYIHAKMRQEHRNRVFHDFRNGLCRNLVCTDLFTRGIDIQAVNVVINFDFPKLAETYLHRIGRSGRFGHLGLAINLITYDDRFNLKSIEEQLGTEIKPIPSNIDKSLYVAEYHSEPVEDEKP
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Molecular Weight
78.87 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
DDX6, a member of the DEAD-box RNA helicase family, plays a crucial role in various cellular processes including RNA metabolism, translation regulation, and stress response. It is known to modulate the stability and translation of specific mRNAs, influencing gene expression and cellular adaptation under different physiological conditions. Dysregulation of DDX6 has been implicated in several diseases, including cancer, neurodegenerative disorders, and viral infections, highlighting its importance in maintaining cellular homeostasis. Research has shown that DDX6 interacts with a variety of RNA-binding proteins and participates in critical cellular complexes such as processing bodies (P-bodies) and stress granules, further emphasizing its role in post-transcriptional regulation. The study of DDX6 recombinant proteins allows researchers to dissect its functional domains, understand its mechanism of action, and explore its potential as a therapeutic target. By examining its interactions and effects on RNA molecules, scientists aim to unravel the complex regulatory networks involving DDX6, paving the way for novel strategies in disease treatment and management.
