Analytical Data
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基因名
MIB1
- Application
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别名
MIB1；DIP1；KIAA1323；ZZANK2；E3 ubiquitin-Protein ligase MIB1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q86YT6
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表达区间
5-332aa
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氨基酸序列
RNNRVMVEGVGARVVRGPDWKWGKQDGGEGHVGTVRSFESPEEVVVVWDNGTAANYRCSGAYDLRILDSAPTGIKHDGTMCDTCRQQPIIGIRWKCAECTNYDLCTVCYHGDKHHLRHRFYRITTPGSERVLLESRRKSKKITARGIFAGARVVRGVDWQWEDQDGGNGRRGKVTEIQDWSASSPHSAAYVLWDNGAKNLYRVGFEGMSDLKCVQDAKGGSFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANIVRSGDAAQGAEGGTSQ
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分子量
40.1kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MIB1 (Mindbomb E3 ubiquitin protein ligase 1) is an E3 ubiquitin ligase that plays a crucial role in the regulation of protein degradation and has been implicated in various cellular processes, including the Notch signaling pathway, which is essential for cell differentiation and development. The study of MIB1 is significant due to its involvement in multiple biological contexts, including embryogenesis, neurogenesis, and cancer progression. Mutations or dysregulation of MIB1 have been associated with tumorigenesis, making it a potential target for therapeutic interventions. Recent research has focused on understanding the molecular mechanisms by which MIB1 regulates substrate ubiquitination and its interactions with other cellular proteins. The production of recombinant MIB1 protein is vital for elucidating its structure-function relationship, as well as for in vitro functional assays to explore its role in ubiquitination and signaling pathways. Advances in recombinant DNA technology and protein expression systems have facilitated the generation of active MIB1, enabling researchers to investigate its enzymatic activity and regulatory roles in cellular contexts. Furthermore, understanding MIB1's function may provide insights into the development of novel cancer therapies and strategies to manipulate cell fate decisions. The investigation of MIB1 continues to be a dynamic area of research with significant implications for both basic biology and clinical applications.
