Analytical Data
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Gene name
NMNAT1
- Application
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Alternative Names
NMNAT1；NMNAT；Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9HAN9
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Expression Region
1-279aa
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AA Sequence
MENSEKTEVVLLACGSFNPITNMHLRLFELAKDYMNGTGRYTVVKGIISPVGDAYKKKGLIPAYHRVIMAELATKNSKWVEVDTWESLQKEWKETLKVLRHHQEKLEASDCDHQQNSPTLERPGRKRKWTETQDSSQKKSLEPKTKAVPKVKLLCGADLLESFAVPNLWKSEDITQIVANYGLICVTRAGNDAQKFIYESDVLWKHRSNIHVVNEWIANDISSTKIRRALRRGQSIRYLVPDLVQEYIEKHNLYSSESEDRNAGVILAPLQRNTAEAKT
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Molecular Weight
58.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Nicotinamide mononucleotide adenylyl transferase 1 (NMNAT1) is a crucial enzyme involved in the biosynthesis of nicotinamide adenine dinucleotide (NAD+), a vital cofactor essential for various cellular processes, including energy metabolism, DNA repair, and cell signaling. NMNAT1 specifically catalyzes the conversion of nicotinamide mononucleotide (NMN) to NAD+, playing a significant role in maintaining cellular NAD+ levels. Recent studies have revealed that NAD+ depletion is associated with various age-related diseases, neurodegenerative disorders, and metabolic dysfunctions, making NMNAT1 a potential target for therapeutic interventions. The recombinant expression of NMNAT1 protein allows for detailed biochemical investigations into its enzymatic activity, regulatory mechanisms, and interaction with other cellular proteins. Understanding the structure-function relationship of NMNAT1 through recombinant studies can aid in the development of NAD+-boosting strategies, including dietary supplements and pharmacological agents, geared towards promoting healthspan and mitigating age-associated decline. Furthermore, the characterization of NMNAT1 could provide insights into its role in diseases such as Alzheimer’s and diabetes, where NAD+ homeostasis is disrupted. Thus, the research on NMNAT1 recombinant protein is pivotal in elucidating the intricate pathways of NAD+ metabolism and exploring novel therapeutic avenues to enhance cellular health and longevity.
