Analytical Data
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Gene name
DCLRE1C
- Application
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Alternative Names
A SCID; A SCID protein; Artemis protein; ASCID; DCLRE1C; DCLRE1C DNA cross link repair 1C ; DCLRE1C protein; DCLREC1C; DCR1C_HUMAN; DNA cross link repair 1C ; DNA cross link repair 1C protein; DNA cross-link repair 1C protein; FLJ11360
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q96SD1
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Expression Region
1-434aa
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AA Sequence
MSSFEGQMAEYPTISIDRFDRENLRARAYFLSHCHKDHMKGLRAPTLKRRLECSLKVYLYCSPVTKELLLTSPKYRFWKKRIISIEIETPTQISLVDEASGEKEEIVVTLLPAGHCPGSVMFLFQGNNGTVLYTGDFRLAQGEAARMELLHSGGRVKDIQSVYLDTTFCDPRFYQIPSREECLSGVLELVRSWITRSPYHVVWLNCKAAYGYEYLFTNLSEELGVQVHVNKLDMFRNMPEILRHLTTDRNTQIHACRHPKAEEYFQWSKLPCGITSRNRIPLHIISIKPSTMWFGERSRKTNVIVRTGESSYRACFSFHSSYSEIKDFLSYLCPVNAYPNVIPVGTTMDKVVEILKPLCRSSQSTEPKYKPLGKLKRARTVHRDSGSHSVTQARMRWCHHDSLYPLTPGIKRSSCLSLLTSWITGAYRHAQLMI
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Molecular Weight
73.48 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
DCLRE1C, also known as Artemis, is a pivotal gene enciphering a protein that is essential for V(D)J recombination, a critical process in the development of functional lymphocytes in the immune system. Mutations in the DCLRE1C gene can lead to severe combined immunodeficiency (SCID), underscoring the importance of Artemis in maintaining genomic stability and facilitating the proper formation of T and B cell receptors. Research on DCLRE1C has gained momentum due to its role in DNA double-strand break repair, particularly in the non-homologous end joining (NHEJ) pathway, which is crucial for repairing genomic lesions. The study of DCLRE1C recombinant protein has allowed scientists to explore its structural and functional properties, contributing to our understanding of its enzymatic mechanisms involved in DNA processing. Additionally, characterizing the recombinant form of Artemis provides invaluable insights into potential therapeutic targets for immunodeficiency disorders and other related conditions. Advances in molecular biology tools and techniques, such as CRISPR-Cas9 and other gene-editing technologies, facilitate the investigation of DCLRE1C's role in cellular and molecular immunology, shedding light on its contributions to immune system function and the implications of its dysfunction. As researchers continue to elucidate the complexities of DCLRE1C, the understanding of its implications for immune dysregulation and potential therapeutic interventions expands, paving the way for innovative approaches in treating immunodeficiencies and related disorders. Overall, the study of DCLRE1C recombinant protein is crucial in advancing our knowledge of immune system development and the underlying mechanisms of genetic disorders associated with its dysfunction.
