Analytical Data
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Gene name
CYP2R1
- Application
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Alternative Names
CP2R1_HUMAN; CYP2R1; Cytochrome P450 2R1; Cytochrome P450 family 2 R1; Cytochrome P450 family 2 subfamily R polypeptide 1; EC 1.14.13.15; MGC4663; Vitamin D 25 hydroxylase; Vitamin D 25-hydroxylase
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q6VVX0
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Expression Region
1-501aa
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AA Sequence
MWKLWRAEEGAAALGGALFLLLFALGVRQLLKQRRPMGFPPGPPGLPFIGNIYSLAASSELPHVYMRKQSQVYGEIFSLDLGGISTVVLNGYDVVKECLVHQSEIFADRPCLPLFMKMTKMGGLLNSRYGRGWVDHRRLAVNSFRYFGYGQKSFESKILEETKFFNDAIETYKGRPFDFKQLITNAVSNITNLIIFGERFTYEDTDFQHMIELFSENVELAASASVFLYNAFPWIGILPFGKHQQLFRNAAVVYDFLSRLIEKASVNRKPQLPQHFVDAYLDEMDQGKNDPSSTFSKENLIFSVGELIIAGTETTTNVLRWAILFMALYPNIQGQVQKEIDLIMGPNGKPSWDDKCKMPYTEAVLHEVLRFCNIVPLGIFHATSEDAVVRGYSIPKGTTVITNLYSVHFDEKYWRDPEVFHPERFLDSSGYFAKKEALVPFSLGRRHCLGEHLARMEMFLFFTALLQRFHLHFPHELVPDLKPRLGMTLQPQPYLICAERR
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Molecular Weight
83.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CYP2R1, a member of the cytochrome P450 superfamily, is primarily known for its role as a vitamin D 25-hydroxylase, which is crucial for the activation of vitamin D in the human body. This enzyme catalyzes the conversion of vitamin D3 (cholecalciferol) into 25-hydroxyvitamin D3 (calcidiol), the major circulating form of vitamin D that is essential for calcium homeostasis and bone health. Research has indicated that variations in the CYP2R1 gene may influence individual responses to vitamin D supplementation and impact overall health, including susceptibility to various diseases. Additionally, understanding the structural and functional properties of CYP2R1 through the study of its recombinant protein can provide insight into its enzymatic mechanisms and regulatory functions. This knowledge is crucial, as deficiencies or dysfunctions in vitamin D metabolism have been associated with conditions such as osteoporosis, autoimmune disorders, and certain cancers. Furthermore, the development of CYP2R1 recombinant protein serves as a valuable tool for drug discovery, enabling the identification of potential therapeutics that can modulate its activity. The study of CYP2R1 not only enhances our understanding of vitamin D metabolism but also opens avenues for research into personalized medicine approaches that can optimize vitamin D-related interventions for diverse populations.
