Analytical Data
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基因名
ABCB11
- Application
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别名
ABCB11；BSEP；Bile salt export pump
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O95342
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表达区间
1033-1321aa
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氨基酸序列
RAFSYTPSYAKAKISAARFFQLLDRQPPISVYNTAGEKWDNFQGKIDFVDCKFTYPSRPDSQVLNGLSVSISPGQTLAFVGSSGCGKSTSIQLLERFYDPDQGKVMIDGHDSKKVNVQFLRSNIGIVSQEPVLFACSIMDNIKYGDNTKEIPMERVIAAAKQAQLHDFVMSLPEKYETNVGSQGSQLSRGEKQRIAIARAIVRDPKILLLDEATSALDTESEKTVQVALDKAREGRTCIVIAHRLSTIQNADIIAVMAQGVVIEKGTHEELMAQKGAYYKLVTTGSPIS
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分子量
35.8 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ABCB11, also known as ATP-binding cassette sub-family B member 11, is a key protein involved in the transport of bile salts and other xenobiotics across cellular membranes. Its primary role is to mediate the hepatocyte secretion of bile acids, crucial for lipid digestion and absorption. Dysregulation or mutations in the ABCB11 gene can lead to a range of hepatic disorders, including progressive familial intrahepatic cholestasis type 2 (PFIC2), which is characterized by cholestasis and liver disease in children. Given the clinical significance of ABCB11, research into its recombinant protein has gained momentum, focusing on understanding its structure, function, and interaction with various ligands. Producing recombinant ABCB11 allows researchers to study its biochemical properties, elucidate the mechanisms of bile acid transport, and assess how mutations affect its function. Furthermore, this research paves the way for potential therapeutic targets aimed at modulating ABCB11 activity for treating related liver diseases. The exploration of ABCB11 not only enhances our understanding of hepatic transport mechanisms but also contributes to the broader field of drug development and precision medicine for bile acid-related disorders. Overall, the study of ABCB11's recombinant protein is critical for advancing both fundamental biology and therapeutic strategies in hepatology.
