Analytical Data
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Gene name
Rbfox3
- Application
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Alternative Names
Rbfox3；RNA binding Protein fox-1 homolog 3
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
A6NFN3
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Expression Region
1-312aa
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AA Sequence
MAQPYPPAQY PPPPQNGIPA EYAPPPPHPT QDYSGQTPVP TEHGMTLYTP AQTHPEQPGS EASTQPIAGT QTVPQTDEAA QTDSQPLHPS DPTEKQQPKR LHVSNIPFRF RDPDLRQMFG QFGKILDVEI IFNERGSKGF GFVTFETSSD ADRAREKLNG TIVEGRKIEV NNATARVMTN KKTGNPYTNG WKLNPVVGAV YGPEFYAVTG FPYPTTGTAV AYRGAHLRGR GRAVYNTFRA APPPPPIPTY GAVVYQDGFY GAEIYGGYAA YRYAQPAAAA AAYSDSYGRV YAAADPYHHT IGPAATYSIG TM
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Molecular Weight
33.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Rbfox3, also known as neuronal splicing regulator 2 (NSR2), is a member of the Rbfox family of proteins, which play critical roles in the regulation of alternative splicing of pre-mRNA, particularly in neuronal tissues. The study of Rbfox3 is significant due to its involvement in essential biological processes, including neuronal development, synaptic plasticity, and the pathophysiology of neurological disorders. Dysregulation of Rbfox3 has been implicated in various conditions such as autism spectrum disorders, schizophrenia, and neurodegenerative diseases, highlighting the importance of understanding its function and mechanism. Research involving the recombinant expression of Rbfox3 protein allows for the investigation of its splicing activity, interaction with RNA targets, and modulation of gene expression. By utilizing recombinant technology, scientists can produce Rbfox3 in a controlled environment, facilitating studies that explore its structural properties, functional domains, and potential therapeutic applications. The insights gained from Rbfox3 research could pave the way for novel strategies in treating neurological diseases where splicing abnormalities play a pivotal role. Overall, the exploration of Rbfox3 recombinant protein holds promise for enhancing our understanding of splicing regulation and its implications in health and disease.
