Analytical Data
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Gene name
bopE
- Application
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Alternative Names
bopE；Guanine nucleotide exchange factor BopE
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q62B14
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Expression Region
1-261aa
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AA Sequence
MTYNPRIGGFTHVKQASFDVHVKRGEAQPRTSFAQQIKRIFSKIGETLGQLFRHRAPDSAPGRVRLQGVRYVGSYRPTGDAKQAIRHFVDEAVKQVAHTRTPEIRQDAEFGRQVYEATLCAIFSEAKDRFCMDPATRAGNVRPAFIEALGDAARATGLPGADKQGVFTPSGAGTNPLYTEIRLRADTLMGAELAARPEYRELQPYARQQAIDLVANALPAERSNTLVEFRQTVQTLEATYRRAAQDASRDEKGATNAADGA
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Molecular Weight
44.7 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
BopE, a multifunctional protein secreted by pathogenic strains of *Burkholderia pseudomallei*, the causative agent of melioidosis, has garnered significant attention in recent years due to its role in modulating host immune responses. As a member of the Type III secretion system (T3SS) effector proteins, BopE plays a crucial role in subverting host defenses and facilitating bacterial virulence. Its ability to manipulate the host's actin cytoskeleton and influence cellular signaling pathways has been linked to enhanced bacterial survival and persistence in host cells. Consequently, understanding the structural and functional properties of BopE is essential for developing novel therapeutic strategies against melioidosis. Researchers have focused on the characterization of BopE through various biochemical and biophysical methods, including X-ray crystallography and mass spectrometry, to elucidate its molecular mechanisms of action. Moreover, recombinant BopE protein is being explored as a potential vaccine candidate, given its immunogenic properties and capacity to provoke a specific immune response. The study of BopE not only aids in comprehending the pathogenicity of *B. pseudomallei*, but also provides insights into the broader field of bacterial pathogenesis, making it a valuable target for both basic and applied microbiological research. As the global burden of melioidosis continues to rise, driven by environmental factors and increasing public health challenges, the investigation of BopE and its functions remains a vital area of interest in infectious disease research.
