Analytical Data
-
Gene name
SFI1
- Application
-
Alternative Names
hSFI1; Protein SFI1 homolog; Sfi1; SFI1_HUMAN
-
Species
Human
-
Source
E. coli
-
Tag
GST-tag at N-terminal
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
A8K8P3
-
Expression Region
1-209 aa
-
AA Sequence
MAQPAAPSLTRPFLAEAPTALVPHSPLPGALSSAPGPKQPPTASTGPELLLLPLSSFMPCGAAAPARVSAQRATPRDKPPVPSSLASVPDPHLLLPGDFSATRAGPGLSTAGSLDLEAELEEIQQQLLHYQTTKQNLWSCRRQASSLRRWLELNREEPGPEDQEVEQQVQKELEQVEMQIQLLAEELQAQRQPIGACVARIQALRQALC
-
Molecular Weight
48.9 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The study of SFI1 recombinant proteins has gained prominence due to their critical role in the assembly and maintenance of the spindle apparatus during cell division. SFI1, or Spindle Formation Integrator 1, is a crucial component that facilitates the interaction between kinetochores and microtubules, ensuring accurate chromosome segregation. Defects in this process can lead to aneuploidy, a condition associated with various cancers and genetic disorders. Understanding the molecular mechanisms governing SFI1's functions can provide insights into the pathways that regulate cell cycle progression and stability. Recent advances in recombinant DNA technology allow for the efficient production of SFI1 proteins, enabling researchers to investigate their structural characteristics, biochemical properties, and interactions with other mitotic proteins. By employing techniques such as X-ray crystallography and cryo-electron microscopy, scientists can elucidate the 3D structures of SFI1 and its complexes, shedding light on how alterations in these interactions may contribute to disease. Furthermore, studies of SFI1-related pathways have implications for developing targeted therapies for cancers characterized by dysregulated cell division. As such, the ongoing research into SFI1 recombinant proteins not only enhances our understanding of fundamental biological processes but also opens new avenues for therapeutic interventions aimed at modulating the cell cycle.
