Analytical Data
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基因名
SMPD1
- Application
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别名
SMPD1；ASM；Sphingomyelin phosphodiesterase
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P17405
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表达区间
47-631aa
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氨基酸序列
LALSDSRVLWAPAEAHPLSPQGHPARLHRIVPRLRDVFGWGNLTCPICKGLFTAINLGLKKEPNVARVGSVAIKLCNLLKIAPPAVCQSIVHLFEDDMVEVWRRSVLSPSEACGLLLGSTCGHWDIFSSWNISLPTVPKPPPKPPSPPAPGAPVSRILFLTDLHWDHDYLEGTDPDCADPLCCRRGSGLPPASRPGAGYWGEYSKCDLPLRTLESLLSGLGPAGPFDMVYWTGDIPAHDVWHQTRQDQLRALTTVTALVRKFLGPVPVYPAVGNHESTPVNSFPPPFIEGNHSSRWLYEAMAKAWEPWLPAEALRTLRIGGFYALSPYPGLRLISLNMNFCSRENFWLLINSTDPAGQLQWLVGELQAAEDRGDKVHIIGHIPPGHCLKSWSWNYYRIVARYENTLAAQFFGHTHVDEFEVFYDEETLSRPLAVAFLAPSATTYIGLNPGYRVYQIDGNYSGSSHVVLDHETYILNLTQANIPGAIPHWQLLYRARETYGLPNTLPTAWHNLVYRMRGDMQLFQTFWFLYHKGHPPSEPCGTPCRLATLCAQLSARADSPALCRHLMPDGSLPEAQSLWPRPLFC
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分子量
78.2 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SMPD1, or sphingomyelin phosphodiesterase 1, is an enzyme critical for sphingolipid metabolism, playing a significant role in the hydrolysis of sphingomyelin to generate ceramide, a signaling molecule involved in various cellular processes, including apoptosis, inflammation, and cellular differentiation. Researchers have increasingly focused on SMPD1 due to its implications in various diseases, particularly neurodegenerative disorders, cancer, and metabolic syndromes. Abnormal SMPD1 activity has been associated with altered lipid metabolism, leading to pathological conditions. Recombinant SMPD1 protein is vital for understanding its structure-function relationships and for identifying potential therapeutic targets. The production of this protein in a recombinant system allows for a detailed investigation of its enzymatic properties and interactions with other biomolecules in vitro. Furthermore, studying the protein's dynamics and regulatory mechanisms could uncover novel insights into its role in disease pathogenesis and offer avenues for drug development. The increasing interest in SMPD1 highlights the necessity of advancing therapeutic strategies that involve modulating its activity to restore normal sphingolipid metabolism. Consequently, research on SMPD1 recombinant protein not only enhances our fundamental understanding of biochemistry but also paves the way for therapeutic innovations in treating related ailments.
