Analytical Data
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基因名
SBP65
- Application
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别名
SBP65;Seed biotin-containing Protein SBP65
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种属
E.coli
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q39846
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表达区间
1-255aa
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氨基酸序列
MASEQLARRENTTTEKEIHVEKHRVPKMATHFEHLAEQAKESDITAGKDTPQGSIEALQAGERVKDHAGKAMGDIGGRGKARETHELGAHFESLADKVTDHAAANVVGNKESQREARGGVRDVGKFEMRTEGGEKGNKDRPELKTRTREVIGRTEKERGRESGGQVVAEKGRETETARGRVGAENEGARTTAVITCTLEKGGGTQKPIREEERESESERSAWEQISNYSDQATQGVKEKYERAKQAASETLNTTT
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分子量
34.9 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SBP65 is a recombinant protein derived from the SBP (Streptavidin-Binding Peptide) that has garnered significant interest in the field of biotechnology and molecular biology. The SBP, originally isolated from *Streptomyces avidinii*, exhibits a strong affinity for biotin, making it an invaluable tool in various applications such as protein labeling, purification, and detection. Researchers have been increasingly exploring SBP65 due to its ability to serve as a versatile scaffold for protein engineering, enabling the development of biosensors and diagnostic tools. The recombinant production of SBP65 allows for the generation of large quantities, facilitating its use in high-throughput systems. Recent studies have focused on optimizing its expression and purification protocols, as well as enhancing its binding properties through mutagenesis. Furthermore, understanding the structural and functional aspects of SBP65 is pivotal for its application in biotechnological innovations, including targeted drug delivery and the creation of biotinylated fusion proteins. As research progresses, the applications of SBP65 could expand, potentially impacting areas such as therapeutics, vaccine development, and the enhancement of scientific research methodologies. Overall, the study of SBP65 represents a significant convergence of protein engineering and molecular recognition, promising to contribute to advances in medical and biological research.












