Analytical Data
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Gene name
CLCN2
- Application
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Alternative Names
Chloride Channel 2; Chloride channel protein 2; Chloride channel; voltage sensitive 2; CIC 2; CIC2; ClC-2; CLC2; Clcn2; CLCN2_HUMAN
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P51788
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Expression Region
38-387aa
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AA Sequence
TVSTAVIVFELTGQIAHILPVMIVVILANAVAQSLQPSLYDSIIRIKKLPYLPELGWGRHQQYRVRVEDIMVRDVPHVALSCTFRDLRLALHRTKGRMLALVESPESMILLGSIERSQVVALLGAQLSPARRRQHMQERRATQTSPLSDQEGPPTPEASVCFQVNTEDSAFPAARGETHKPLKPALKRGPSVTRNLGESPTGSAESAGIALRSLFCGSPPPEAASEKLESCEKRKLKRVRISLASDADLEGEMSPEETHTIFSLLGVDHAYVTSIGRLIGIVTLKELRKAIEGSVTAQGVKVRPPLASFRDSATSSSDTETTEVHALWGPHSRHGLPREGSPSDSDDKCQ
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Molecular Weight
64.24 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CLCN2, a member of the chloride channel family, plays a crucial role in maintaining cellular ion homeostasis and has been implicated in various physiological processes, including neuronal excitability and the regulation of transepithelial transport. Mutations in the CLCN2 gene have been linked to several neurological disorders, making it a subject of intense research. The ability to study CLCN2 through recombinant protein expression has opened new avenues for understanding its structure-function relationships, ion conduction mechanisms, and potential pharmacological interventions. By producing and purifying CLCN2 as a recombinant protein, researchers can perform functional assays, electrophysiological studies, and crystallography to unveil the molecular basis of its action. This research is vital not only for illuminating the underlying causes of CLCN2-related pathologies but also for developing targeted therapies that may alleviate symptoms associated with these disorders. Furthermore, the study of CLCN2 contributes to the broader understanding of chloride channels in health and disease, offering insights into their regulatory roles in various tissues and potential therapeutic targets for related conditions.
