Analytical Data
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Gene name
NR1H3
- Application
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Alternative Names
NR1H3；LXRA；Oxysterols receptor LXR-alpha
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q13133
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Expression Region
1-402aa
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AA Sequence
MPHSAGGTAGVGLEAAEPTALLTRAEPPSEPTEIRPQKRKKGPAPKMLGNELCSVCGDKASGFHYNVLSCEGCKGFFRRSVIKGAHYICHSGGHCPMDTYMRRKCQECRLRKCRQAGMREECVLSEEQIRLKKLKRQEEEQAHATSLPPRASSPPQILPQLSPEQLGMIEKLVAAQQQCNRRSFSDRLRVTPWPMAPDPHSREARQQRFAHFTELAIVSVQEIVDFAKQLPGFLQLSREDQIALLKTSAIEVMLLETSRRYNPGSESITFLKDFSYNREDFAKAGLQVEFINPIFEFSRAMNELQLNDAEFALLIAISIFSADRPNVQDQLQVERLQHTYVEALHAYVSIHHPHDRLMFPRMLMKLVSLRTLSSVHSEQVFALRLQDKKLPPLLSEIWDVHE
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Molecular Weight
53.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NR1H3, also known as Liver X Receptor Alpha (LXRα), is a nuclear receptor that plays a crucial role in the regulation of lipid metabolism, cholesterol homeostasis, and inflammatory responses. Research into NR1H3 has gained significant attention due to its potential implications in various metabolic disorders, including obesity, atherosclerosis, and diabetes. LXRα functions primarily by regulating the expression of genes involved in lipogenesis, cholesterol efflux, and inflammation, thus serving as a key player in maintaining cellular and systemic lipid balance. Studies have demonstrated that activation of NR1H3 can lead to increased reverse cholesterol transport, promoting the clearance of excess cholesterol from peripheral tissues to the liver for excretion. This mechanism positions LXRα as a promising therapeutic target for cardiovascular diseases. Moreover, its role in modulating inflammatory pathways highlights its potential in treating metabolic syndrome and associated complications. Advances in recombinant protein technology have enabled the production of functional NR1H3, facilitating in vitro and in vivo studies. Characterizing the structural and functional properties of this receptor not only enhances our understanding of its mechanisms of action but also assists in the development of selective agonists or antagonists, paving the way for novel therapeutic strategies targeting metabolic and inflammatory diseases. Overall, ongoing research on NR1H3 recombinant protein underscores its significance in metabolic regulation and its promise as a target for innovative treatments in metabolic disorders.
