Analytical Data
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基因名
AGO2
- Application
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别名
AGO2；EIF2C2；Protein argonaute-2
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9UKV8
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表达区间
517-818aa
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氨基酸序列
LVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLV
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分子量
36.1kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
AGO2, or Argonaute 2, is a key protein involved in the RNA interference (RNAi) pathway, playing a crucial role in post-transcriptional gene regulation by binding to small RNA molecules such as microRNAs (miRNAs) and small interfering RNAs (siRNAs). Its discovery has significantly advanced our understanding of gene silencing mechanisms, as AGO2 is responsible for loading these small RNAs and guiding them to their target messenger RNAs (mRNAs), ultimately leading to mRNA degradation or translational repression. Research on AGO2 recombinant protein is pivotal for elucidating its structural and functional properties, enabling scientists to explore the molecular basis of RNAi and its applications in therapeutic contexts, such as targeted gene therapy and cancer treatment. Given its central role in gene regulation, recombinant AGO2 can be utilized to investigate miRNA-mRNA interactions, assess the efficiency of RNAi processes, and develop novel strategies for manipulating gene expression in various biological systems. Additionally, understanding the protein-protein interactions involving AGO2 may reveal new regulatory networks essential for cellular processes and disease states. The study of recombinant AGO2 not only provides insights into fundamental biological mechanisms but also holds promise for developing innovative approaches to combat genetic disorders and enhance biotechnological applications.
