Analytical Data
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Gene name
walK
- Application
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Alternative Names
walK；SEMAN；SEMAZ；Semaphorin-6B
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
A6QD58
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Expression Region
382-600aa
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AA Sequence
NVSHELRTPLTSMNSYIEALEEGAWKDEELAPQFLSVTREETERMIRLVNDLLQLSKMDNESDQINKEIIDFNMFINKIINRHEMSAKDTTFIRDIPKKTIFTEFDPDKMTQVFDNVITNAMKYSRGDKRVEFHVKQNPLYNRMTIRIKDNGIGIPINKVDKIFDRFYRVDKARTRKMGGTGLGLAISKEIVEAHNGRIWANSVEGQGTSIFITLPCEV
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Molecular Weight
29.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The WALK protein, originally identified in studies focusing on cellular signaling and development, plays a critical role in various physiological processes. It is part of a larger family of proteins involved in signal transduction pathways that regulate cell growth, differentiation, and apoptosis. Researchers have been particularly interested in the WALK protein due to its involvement in cellular response to environmental stimuli and its potential implications in disease mechanisms. For instance, aberrations in WALK function have been linked to certain cancers and developmental disorders, making it a target for therapeutic interventions. Moreover, advances in recombinant protein technology have enabled scientists to produce WALK in a laboratory setting, facilitating detailed structural and functional analyses. This has spurred a deeper understanding of its interactions with other proteins and its role within larger cellular networks. As such, WALK represents a valuable biomolecule for both basic research and potential clinical applications, underscoring the need for ongoing studies to elucidate its mechanisms of action and therapeutic potential.
