Analytical Data
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基因名
amiF
- Application
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别名
amiF；Formamidase
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种属
Bradyrhizobium
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
A4Z3G9
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表达区间
1-337aa
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氨基酸序列
MNGLGGLNKSEHGVVIGLVQLQLPVVVTKEDLAKQTEKIVWMVGKARRNLGTMDLVVFPEYSLHGLSMDTNPEIMCRLDGPEVAAFKQACIDNKIWGCFSIMEYNPDGNPYNSGLIIDSNGEIKLYYRKLHPWIPVEPWEPGDLGIPVIEGPRGAKIALIICHDGMFPEMARECAYKGAEIMIRTAGYTAPIRDSWRFTNQANAFQNLMVTANVCMCGSDGSFDSMGEGMIVNFDGSILAHGTTGRADEIITAEVRPDLVREARIGWGVENNIYQLWHRGYVAVKGGAMDCPYTFMHDMVAGTYRLPWEDQVKITDGTSCGFPAPTRVFGKMAKAAE
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分子量
53.2 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of amiF recombinant proteins has gained significant attention due to their potential applications in various biotechnological and pharmaceutical fields. amiF, a gene encoding for an amidohydrolase enzyme, is involved in the hydrolysis of amides, playing a crucial role in metabolic pathways across different organisms. Understanding the structure and function of amiF can provide insights into its catalytic mechanisms, which are essential for designing enzyme inhibitors or enhancing enzyme activity for industrial applications. Furthermore, the production of amiF recombinant proteins allows for the exploration of their properties in controlled environments, facilitating the development of improved biocatalysts. As industries increasingly seek sustainable and efficient processes, the ability to harness and modify these enzymes can lead to breakthroughs in bioprocessing and drug development. The exploration of amiF in therapeutic contexts, particularly for its potential use in treating conditions linked to amide metabolism, illustrates the relevance of this research. Overall, the investigation of amiF recombinant proteins holds promise for advancing our understanding of enzymatic functions and developing innovative solutions for pressing challenges in health and biotechnology.
