Analytical Data
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Gene name
deoD
- Application
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Alternative Names
deoD；pup；Purine nucleoside phosphorylase DeoD-type
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P0ABP8
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Expression Region
2-239aa
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AA Sequence
ATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVRGMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLRDVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLFYSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAERQTTFNDMIKIALESVLLGDKE
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Molecular Weight
52.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
DeoD is a key enzyme involved in the metabolism of purines, playing a crucial role in the salvage pathway of nucleoside triphosphates. Its activity is essential for maintaining the nucleotide pool within cells, which is vital for DNA and RNA synthesis. Research on DeoD has gained momentum due to its potential implications in various biological processes and diseases, including cancer, where altered purine metabolism can contribute to tumor growth and resistance to therapies. The production of recombinant DeoD protein has become a focus of biotechnological studies to better understand its structure-function relationship, regulatory mechanisms, and interactions with other metabolic pathways. Advances in molecular cloning and protein expression techniques enable researchers to obtain large quantities of purified DeoD, facilitating detailed biochemical characterization and potential therapeutic applications. In this context, insights into the enzyme's kinetics, substrate specificity, and inhibition could pave the way for developing novel strategies to manipulate purine metabolism for therapeutic benefits. Overall, the study of recombinant DeoD not only enhances our understanding of purine metabolism but also opens new avenues for targeted drug development and metabolic engineering.
