Analytical Data
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Gene name
ECE1
- Application
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Alternative Names
ECE1；Endothelin-converting enzyme 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P42892
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Expression Region
90-770aa
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AA Sequence
QYQTRSPSVC LSEACVSVTS SILSSMDPTV DPCHDFFSYA CGGWIKANPV PDGHSRWGTF SNLWEHNQAI IKHLLENSTA SVSEAERKAQ VYYRACMNET RIEELRAKPL MELIERLGGW NITGPWAKDN FQDTLQVVTA HYRTSPFFSV YVSADSKNSN SNVIQVDQSG LGLPSRDYYL NKTENEKVLT GYLNYMVQLG KLLGGGDEEA IRPQMQQILD FETALANITI PQEKRRDEEL IYHKVTAAEL QTLAPAINWL PFLNTIFYPV EINESEPIVV YDKEYLEQIS TLINTTDRCL LNNYMIWNLV RKTSSFLDQR FQDADEKFME VMYGTKKTCL PRWKFCVSDT ENNLGFALGP MFVKATFAED SKSIATEIIL EIKKAFEESL STLKWMDEET RKSAKEKADA IYNMIGYPNF IMDPKELDKV FNDYTAVPDL YFENAMRFFN FSWRVTADQL RKAPNRDQWS MTPPMVNAYY SPTKNEIVFP AGILQAPFYT RSSPKALNFG GIGVVVGHEL THAFDDQGRE YDKDGNLRPW WKNSSVEAFK RQTECMVEQY SNYSVNGEPV NGRHTLGENI ADNGGLKAAY RAYQNWVKKN GAEHSLPTLG LTNNQLFFLG FAQVWCSVRT PESSHEGLIT DPHSPSRFRV IGSLSNSKEF SEHFRCPPGS PMNPPHKCEV W
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Molecular Weight
80 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ECE1 (Endothelin-converting enzyme 1) is a critical membrane-bound protease involved in the processing of pre-proendothelin into biologically active endothelin peptides, which are potent vasoconstrictors and regulators of cardiovascular homeostasis and cellular growth. Dysregulation of ECE1 activity is implicated in various pathological conditions, including hypertension, heart failure, and cancer, making it a significant target for therapeutic intervention. Research into ECE1 has gained momentum due to its role in endothelial function and its contribution to the endothelin system, which is essential for vascular biology. Advances in molecular biology and biochemistry have facilitated the study of ECE1's structure, activity, and regulatory mechanisms. The development of recombinant ECE1 proteins has allowed researchers to investigate the enzyme's enzymatic properties, substrate specificity, and potential inhibitors in greater detail. Furthermore, understanding the role of ECE1 in disease mechanisms opens up new avenues for drug discovery, as ECE1 inhibitors could serve as novel therapeutic agents. Overall, the ongoing research into ECE1 not only enhances our understanding of cardiovascular physiology but also paves the way for innovative strategies to manage diseases linked to the endothelin system.
