Analytical Data
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Gene name
CCT5
- Application
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Alternative Names
CCT 5; CCT epsilon ; CCT-epsilon; CCT5; CCTE; CCTepsilon; Chaperonin containing T-complex polypeptide 1; subunit 5; Chaperonin containing TCP 1 epsilon; Chaperonin containing TCP 1 subunit 5; Chaperonin containing TCP 1 subunit 5 epsilon
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P48643
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Expression Region
1-541aa
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AA Sequence
MASMGTLAFDEYGRPFLIIKDQDRKSRLMGLEALKSHIMAAKAVANTMRTSLGPNGLDKMMVDKDGDVTVTNDGATILSMMDVDHQIAKLMVELSKSQDDEIGDGTTGVVVLAGALLEEAEQLLDRGIHPIRIADGYEQAARVAIEHLDKISDSVLVDIKDTEPLIQTAKTTLGSKVVNSCHRQMAEIAVNAVLTVADMERRDVDFELIKVEGKVGGRLEDTKLIKGVIVDKDFSHPQMPKKVEDAKIAILTCPFEPPKPKTKHKLDVTSVEDYKALQKYEKEKFEEMIQQIKETGANLAICQWGFDDEANHLLLQNNLPAVRWVGGPEIELIAIATGGRIVPRFSELTAEKLGFAGLVQEISFGTTKDKMLVIEQCKNSRAVTIFIRGGNKMIIEEAKRSLHDALCVIRNLIRDNRVVYGGGAAEISCALAVSQEADKCPTLEQYAMRAFADALEVIPMALSENSGMNPIQTMTEVRARQVKEMNPALGIDCLHKGTNDMKQQHVIETLIGKKQQISLATQMVRMILKIDDIRKPGESEE
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Molecular Weight
85.25 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CCT5, a member of the chaperonin-containing TCP1 (CCT) complex, plays a crucial role in protein folding and assembly within the cytosol of eukaryotic cells. This complex assists in the proper folding of various structural proteins, including actins and tubulins, which are essential for maintaining cellular integrity and function. Research on CCT5 is driven by its potential implications in diseases associated with protein misfolding and aggregation, such as neurodegenerative disorders and cancer. Understanding the mechanistic intricacies of CCT5 can provide insights into its function in cellular homeostasis and stress responses. Moreover, the recombinant expression of CCT5 can facilitate the study of its structure-function relationship and its interactions with substrate proteins, ultimately contributing to the development of therapeutic strategies aimed at modulating protein folding pathways. Additionally, given the evolutionarily conserved nature of CCT proteins, findings from CCT5 research may extend to broader biological contexts, enhancing our comprehension of proteomic stability and cellular resilience across different species. Therefore, investigating CCT5 as a recombinant protein not only emphasizes its biochemical significance but also underscores the potential for innovating treatment modalities for protein misfolding-related diseases.
