Analytical Data
-
基因名
plcH
- Application
-
别名
plcH；KIAA1069；PLCL3；1-phosphatidylinositol 4.5-bisphosphate phosphodiesterase eta-1
-
种属
Human
-
表达系统
E. coli
-
标签
His tag N-Terminus
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
P06200
-
表达区间
51-436aa
-
氨基酸序列
VQHVVILMQENRSFDHYFGHLNGVRGFNDPRALKRQDGKPVWYQNYKYEF SPYHWDTKVTSAQWVSSQNHEWSAFHAIWNQGRNDKWMAVQYPEAMGYFK RGDIPYYYALADAFTLCEAYHQSMMGPTNPNRLYHMSGRAAPSGDGKDVH IGNDMGDGTIGASGTVDWTTYPERLSAAGVDWRVYQEGGYRSSSLWYLYV DAYWKYRLQEQNNYDCNALAWFRNFKNAPRDSDLWQRAMLARGVDQLRKD VQENTLPQVSWIVAPYCYCEHPWWGPSFGEYYVTRVLEALTSNPEVWART VFILNYDEGDGFYDHASAPVPPWKDGVGLSTVSTAGEIEVSSGLPIGLGH RVPLIAISPWSKGGKVSAEVFDHTSVLRFLERRFGV
-
分子量
64 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PLC-γ (phospholipase C-gamma) is an important enzyme that plays a critical role in cellular signaling pathways by hydrolyzing phosphatidylinositol 4,5-bisphosphate to generate inositol trisphosphate and diacylglycerol. This process is essential for various physiological functions, including cell proliferation, differentiation, and migration. The study of PLC-γ recombinant proteins has gained significant attention due to their implications in cancer biology and other diseases. Overexpression or dysregulation of PLC-γ is often observed in various cancers, making it a potential therapeutic target. Moreover, recombinant PLC-γ proteins can be utilized to dissect the mechanistic pathways of signal transduction and to develop new pharmacological agents. Understanding the structural and functional aspects of PLC-γ through recombinant technology enables researchers to design specific inhibitors and antibodies that can modulate its activity. This research is not only pivotal for advancing cancer treatment but also for expanding our understanding of complex cellular processes. As a result, the production and characterization of PLC-γ recombinant proteins have become a significant area of focus in biochemistry and molecular biology, contributing to the development of targeted therapies and personalized medicine approaches.
