Analytical Data
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Gene name
plcH
- Application
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Alternative Names
plcH；KIAA1069；PLCL3；1-phosphatidylinositol 4.5-bisphosphate phosphodiesterase eta-1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P06200
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Expression Region
51-436aa
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AA Sequence
VQHVVILMQENRSFDHYFGHLNGVRGFNDPRALKRQDGKPVWYQNYKYEF SPYHWDTKVTSAQWVSSQNHEWSAFHAIWNQGRNDKWMAVQYPEAMGYFK RGDIPYYYALADAFTLCEAYHQSMMGPTNPNRLYHMSGRAAPSGDGKDVH IGNDMGDGTIGASGTVDWTTYPERLSAAGVDWRVYQEGGYRSSSLWYLYV DAYWKYRLQEQNNYDCNALAWFRNFKNAPRDSDLWQRAMLARGVDQLRKD VQENTLPQVSWIVAPYCYCEHPWWGPSFGEYYVTRVLEALTSNPEVWART VFILNYDEGDGFYDHASAPVPPWKDGVGLSTVSTAGEIEVSSGLPIGLGH RVPLIAISPWSKGGKVSAEVFDHTSVLRFLERRFGV
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Molecular Weight
64 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PLC-γ (phospholipase C-gamma) is an important enzyme that plays a critical role in cellular signaling pathways by hydrolyzing phosphatidylinositol 4,5-bisphosphate to generate inositol trisphosphate and diacylglycerol. This process is essential for various physiological functions, including cell proliferation, differentiation, and migration. The study of PLC-γ recombinant proteins has gained significant attention due to their implications in cancer biology and other diseases. Overexpression or dysregulation of PLC-γ is often observed in various cancers, making it a potential therapeutic target. Moreover, recombinant PLC-γ proteins can be utilized to dissect the mechanistic pathways of signal transduction and to develop new pharmacological agents. Understanding the structural and functional aspects of PLC-γ through recombinant technology enables researchers to design specific inhibitors and antibodies that can modulate its activity. This research is not only pivotal for advancing cancer treatment but also for expanding our understanding of complex cellular processes. As a result, the production and characterization of PLC-γ recombinant proteins have become a significant area of focus in biochemistry and molecular biology, contributing to the development of targeted therapies and personalized medicine approaches.
