Analytical Data
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基因名
repD
- Application
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别名
repD；Replication initiation Protein
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种属
Staphylococcus aureus
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P03065
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表达区间
1-311aa
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氨基酸序列
MSTENHSNYLQNKDLDNFSKTGYSNSRLSGNFFTTPQPELSFDAMTIVGNLNKTNAKKLSDFMSTEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADSWDRRNMRVEFNPNKLTHEEMLWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKAVKKTIFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVMSEHLWRVEIELKRDMVDYWNDCFDDLHILKPDWTTPEKVKEQAMVYLLLNEEGTWGKLERHAKYKYKQLIKEISPIDLTELMKSTLKENEKQLQKQIDFWQREFRFWK
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分子量
53.5 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RepD is a crucial protein implicated in the replication and maintenance of plasmids in various bacterial species, particularly in those carrying a broad-host-range plasmid. It belongs to the helicase family and plays a vital role in unwinding the DNA during the replication process. Understanding RepD is essential due to its potential applications in biotechnology and genetic engineering, where plasmid-based systems are commonly used for gene cloning, expression, and manipulation. Recent studies have focused on elucidating the biochemical and structural properties of RepD, aiming to reveal its mechanistic action and interaction with DNA. Additionally, the role of RepD in plasmid stability and its response to environmental stresses has garnered interest, as this knowledge could enhance the production of recombinant proteins and improve the efficacy of plasmid vectors. Enhanced expression systems utilizing RepD could revolutionize industrial microbiology and synthetic biology by facilitating more robust and efficient plasmid-based tools for gene delivery and manipulation. Therefore, ongoing research into RepD not only contributes to our fundamental understanding of plasmid biology but also opens avenues for innovative applications in biotechnology and medicine.
