Analytical Data
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Gene name
ALDH1A3
- Application
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Alternative Names
ALDH1A3；ALDH6；Retinaldehyde dehydrogenase 3
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P47895
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Expression Region
2-512aa
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AA Sequence
ATANGAVENGQPDRKPPALPRPIRNLEVKFTKIFINNEWHESKSGKKFATCNPSTREQICEVEEGDKPDVDKAVEAAQVAFQRGSPWRRLDALSRGRLLHQLADLVERDRATLAALETMDTGKPFLHAFFIDLEGCIRTLRYFAGWADKIQGKTIPTDDNVVCFTRHEPIGVCGAITPWNFPLLMLVWKLAPALCCGNTMVLKPAEQTPLTALYLGSLIKEAGFPPGVVNIVPGFGPTVGAAISSHPQINKIAFTGSTEVGKLVKEAASRSNLKRVTLELGGKNPCIVCADADLDLAVECAHQGVFFNQGQCCTAASRVFVEEQVYSEFVRRSVEYAKKRPVGDPFDVKTEQGPQIDQKQFDKILELIESGKKEGAKLECGGSAMEDKGLFIKPTVFSEVTDNMRIAKEEIFGPVQPILKFKSIEEVIKRANSTDYGLTAAVFTKNLDKALKLASALESGTVWINCYNALYAQAPFGGFKMSGNGRELGEYALAEYTEVKTVTIKLGDKNP
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Molecular Weight
58.0kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Aldehyde dehydrogenase 1A3 (ALDH1A3) is an enzyme that plays a crucial role in the oxidation of aldehydes to carboxylic acids, thus participating in various metabolic processes, including retinoic acid metabolism, which is vital for embryonic development and differentiation. Recent studies have highlighted the importance of ALDH1A3 in cancer research, particularly in the context of stem cell biology and tumorigenesis, as elevated levels of this enzyme are often associated with poor prognosis in several cancers. Additionally, ALDH1A3 is implicated in the detoxification of aldehyde metabolites that can cause cellular damage; hence, its study may unveil potential therapeutic targets for a range of diseases. The recombinant production of ALDH1A3 allows for detailed biochemical characterization and provides insights into its structure-function relationships, facilitating drug design and development. By using recombinant techniques, researchers can obtain large quantities of pure enzyme for experimental analysis, including substrate specificity, inhibition studies, and the effects of mutations on enzyme activity. Understanding the regulation and activity of ALDH1A3 can shed light on its role in normal physiology and in pathological conditions, making it a promising subject for further research in both basic and applied sciences.
