Analytical Data
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Gene name
RNF135
- Application
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Alternative Names
E3 ubiquitin-protein ligase RNF135; L13; MGC13061; MMFD; REUL; RIG-I E3 ubiquitin ligase; RING finger protein 135; Riplet; RN135_HUMAN; Rnf135
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8IUD6
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Expression Region
1-432 aa
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AA Sequence
MAGLGLGSAV PVWLAEDDLG CIICQGLLDW PATLPCGHSF CRHCLEALWG ARDARRWACP TCRQGAAQQP HLRKNTLLQD LADKYRRAAR EIQAGSDPAH CPCPGSSSLS SAAARPRRRP ELQRVAVEKS ITEVAQELTE LVEHLVDIVR SLQNQRPLSE SGPDNELSIL GKAFSSGVDL SMASPKLVTS DTAAGKIRDI LHDLEEIQEK LQESVTWKEA PEAQMQGELL EAPSSSSCPL PDQSHPALRR ASRFAQWAIH PTFNLKSLSC SLEVSKDSRT VTVSHRPQPY RWSCERFSTS QVLCSQALSS GKHYWEVDTR NCSHWAVGVA SWEMSRDQVL GRTMDSCCVE WKGTSQLSAW HMVKETVLGS DRPGVVGIWL NLEEGKLAFY SVDNQEKLLY ECTISASSPL YPAFWLYGLH PGNYLIIKQV KV
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Molecular Weight
47.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RNF135 is an E3 ubiquitin ligase that has garnered scientific interest due to its role in the regulation of various cellular processes, including protein degradation, cell cycle control, and immune responses. Dysregulation of RNF135 has been implicated in several diseases, including cancer and autoimmune disorders, highlighting its potential as a therapeutic target. Recent studies have shown that RNF135 modulates the degradation of key signaling molecules, thereby influencing pathways such as NF-kB and p53, which are critical for cell proliferation and survival. Additionally, RNF135 has been linked to the regulation of inflammatory responses, making it a significant player in maintaining immune homeostasis. Understanding the structure and function of RNF135, along with its interactions with other proteins, is essential for elucidating its roles in health and disease. Thus, the study of recombinant RNF135 protein is crucial for dissecting its biological functions, exploring its involvement in disease mechanisms, and developing potential therapeutic interventions aimed at targeting its activity. This research framework not only aids in clarifying the molecular pathways involving RNF135 but also contributes to the broader understanding of ubiquitin-mediated signaling processes in cellular biology.
