Analytical Data
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基因名
rpmA
- Application
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别名
rpmA；Large ribosomal subunit Protein bL27
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种属
E.coli
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P0A7L8
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表达区间
2-85aa
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氨基酸序列
AHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTKFHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE
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分子量
36.0 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of the rpmA gene, which encodes the ribosomal protein L27 in bacteria, is significant due to its crucial role in protein synthesis and ribosome function. Ribosomal proteins play essential roles in the assembly, structural integrity, and overall functionality of ribosomes, the cellular machinery responsible for translating mRNA into proteins. Intriguingly, mutations or deletions in the rpmA gene can lead to defective ribosome assembly, impacting cellular growth and viability. Given the rising issue of antibiotic resistance, understanding the biochemical mechanisms of ribosomal proteins like RpmA can provide insights into developing novel antibiotics targeting bacterial protein synthesis. Furthermore, recombinant RpmA protein can be produced and studied to elucidate its structural characteristics and interactions within the ribosomal context. This research not only enhances our comprehension of bacterial ribosome assembly but also sets the foundation for potential therapeutic strategies against pathogenic bacteria. By exploring the functional dynamics of RpmA, researchers aim to contribute to the broader field of microbiology and antibiotic development, addressing critical challenges in infectious disease management.
