Analytical Data
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Gene name
RIT1
- Application
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Alternative Names
GTP binding protein Roc1; GTP-binding protein Rit1; NS8; Ras like protein expressed in many tissues; Ras like without CAAX 1; Ras-like protein expressed in many tissues; Ras-like without CAAX protein 1; RIBB; Ric like expressed in many tissues; Ric-like protein without CAAX motif 1; RIT; RIT1; RIT1_HUMAN; ROC1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q92963
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Expression Region
1-219 aa
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AA Sequence
MDSGTRPVGS CCSSPAGLSR EYKLVMLGAG GVGKSAMTMQ FISHRFPEDH DPTIEDAYKI RIRIDDEPAN LDILDTAGQA EFTAMRDQYM RAGEGFIICY SITDRRSFHE VREFKQLIYR VRRTDDTPVV LVGNKSDLKQ LRQVTKEEGL ALAREFSCPF FETSAAYRYY IDDVFHALVR EIRRKEKEAV LAMEKKSKPK NSVWKRLKSP FRKKKDSVT
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Molecular Weight
25.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RIT1 is a member of the Ras superfamily of small GTPases and plays a critical role in various cellular processes, including signal transduction and cytoskeletal dynamics. Recent studies have implicated RIT1 in various human diseases, particularly in cancer, where its mutations or dysregulation contribute to tumorigenesis. RIT1 is involved in the regulation of cellular growth and differentiation, and its aberrant signaling can lead to uncontrolled cell proliferation. Researchers have focused on the recombinant expression of RIT1 to better understand its functional mechanisms and interactions within the cell. The generation of recombinant RIT1 protein allows for detailed biochemical and biophysical characterization, enabling scientists to investigate its role in specific signaling pathways and its potential as a therapeutic target. Furthermore, the study of RIT1’s structure and function can reveal insights into its regulatory mechanisms and how it communicates with various effector proteins. The ongoing research aims to elucidate the precise biological role of RIT1, explore its involvement in disease processes, and assess its potential as a biomarker for certain cancers or as a target for drug development. Understanding RIT1's function and interactions could pave the way for novel therapeutic strategies aimed at mitigating the effects of its dysregulation in diseases.
