Cat: PA2000-2782

Recombinant E.coli ompT Protein,His

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Analytical Data

  • 基因名

    ompT

  • Application

    SPRMSTBLIITCELISA细胞实验药物筛选

  • 别名

    ompT;Protease 7

  • 种属

    E.coli

  • 表达系统

    E. coli

  • 标签

    His tag N-Terminus

  • 纯度

    Greater than 90% as determined by SDS-PAGE.

  • 蛋白编号

    P09169

  • 表达区间

    21-317aa

  • 氨基酸序列

    STETLSFTPDNINADISLGTLSGKTKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMVDQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYSFTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTFKYSGWVESSDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRVTNKKGNTSLYDHNNNTSDYSKNGAGIENYNFITTAGLKYTF

  • 分子量

    37.5 kDa

  • 内毒素

    < 1.0 EU per μg protein as determined by the LAL method.

  • 性状

    Freeze-dried powder

  • 缓冲液

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • 复溶方法

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • 个性化定制

    点位突变 标签定制 buffer定制 全长蛋白定制

  • 稳定性测试

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • 保存条件 & 期限

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • 运输条件

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

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Protein Description

The ompT protein, a member of the outer membrane protease family found in Gram-negative bacteria, plays a crucial role in various biological processes, including protein quality control and the maintenance of outer membrane integrity. Its significance has gained attention due to its involvement in the degradation of misfolded or damaged proteins, which helps bacteria adapt to environmental stresses. Researchers have increasingly focused on the recombinant expression of ompT for several reasons. Firstly, it has potential applications in biotechnology and medicine, such as in the development of biosensors or therapeutic proteins. Additionally, understanding the structure and function of ompT can provide insights into bacterial pathogenicity and resistance mechanisms. The challenges associated with successfully obtaining and characterizing recombinant ompT, such as ensuring proper folding and activity in a heterologous expression system, have spurred advancements in protein engineering and expression techniques. Studies have shown that optimizing expression conditions and employing suitable tags can enhance yield and functionality of ompT. Consequently, research on ompT recombinant protein not only furthers our understanding of bacterial physiology but also opens avenues for innovations in antimicrobial strategies and biotechnological applications. This research area continues to evolve, driven by the need for efficient protein production systems and the rising importance of outer membrane proteins in microbial ecology and pathogenicity.

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IPODIX North America (HQ)
Proteintech Group, Inc
5500 Pearl Street, Suite 400
Rosemont, IL 60018, USA
1-888-478-4522
proteintech@ptglab.com
IPODIX North America (HQ)
Proteintech Group, Inc
5500 Pearl Street, Suite 400
Rosemont, IL 60018, USA
1-888-478-4522
proteintech@ptglab.com
IPODIX North America (HQ)
Proteintech Group, Inc
5500 Pearl Street, Suite 400
Rosemont, IL 60018, USA
1-888-478-4522
proteintech@ptglab.com
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