Analytical Data
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基因名
isaA
- Application
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别名
isaA；Probable transglycosylase IsaA
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种属
E.coli
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P60157
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表达区间
30-233aa
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氨基酸序列
AEVNVDQAHLVDLAHNHQDQLNAAPIKDGAYDIHFVKDGFQYNFTSNGTTWSWSYEAANGQTAGFSNVAGADYTTSYNQGSNVQSVSYNAQSSNSNVEAVSAPTYHNYSTSTTSSSVRLSNGNTAGATGSSAAQIMAQRTGVSASTWAAIIARESNGQVNAYNPSGASGLFQTMPGWGPTNTVDQQINAAVKAYKAQGLGAWGF
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分子量
28.8 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of isaA recombinant protein is rooted in its significant role in bacterial physiology and pathogenesis, particularly in the context of the bacterium *Streptococcus pneumoniae*. IsaA, or Inducible ginA, is a protein that has been implicated in the bacteria's ability to evade the host immune response and enhance virulence. Understanding the structure and function of isaA is crucial for deciphering its involvement in disease mechanisms, as well as for potential applications in vaccine development or therapeutic interventions. The interest in recombinant versions of this protein arises from the need to investigate its biochemical properties and interactions within the host, which can provide insights into bacterial behavior and potential vulnerabilities. Researchers employ techniques such as molecular cloning and protein expression systems to produce and characterize isaA, leading to a better understanding of its role in the pathogenicity of *S. pneumoniae*. Ultimately, this research may contribute to efforts aimed at combating infections caused by this significant human pathogen.
