Cat: PA2000-6434

Recombinant Human CAPZA1 Protein,GST

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Analytical Data

  • Gene name

    CAPZA1

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    Cap Z; Cappa 1; Cappa1; Capping Protein (actin filament) muscle Z line alpha 1; Capping Protein alpha 1; Capping Protein muscle Z line alpha 1; CapZ alpha 1; CapZ alpha-1; CAPZ; CAPZA 1

  • Species

    Human

  • Source

    E. coli

  • Tag

    GST-tag at N-terminal

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    P52907

  • Expression Region

    1-286aa

  • AA Sequence

    MADFDDRVSDEEKVRIAAKFITHAPPGEFNEVFNDVRLLLNNDNLLREGAAHAFAQYNMDQFTPVKIEGYEDQVLITEHGDLGNSRFLDPRNKISFKFDHLRKEASDPQPEEADGGLKSWRESCDSALRAYVKDHYSNGFCTVYAKTIDGQQTIIACIESHQFQPKNFWNGRWRSEWKFTITPPTAQVVGVLKIQVHYYEDGNVQLVSHKDVQDSLTVSNEAQTAKEFIKIIENAENEYQTAISENYQTMSDTTFKALRRQLPVTRTKIDWNKILSYKIGKEMQNA

  • Molecular Weight

    57.2 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

Quality inspection process

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Protein Description

CAPZA1, or Cap-zeta, is a member of the CAPZ family of proteins that play a crucial role in regulating actin dynamics and cellular structures. Actin filaments are fundamental components of the cytoskeleton, influencing cell shape, motility, and division. CAPZA1 functions as a capping protein that binds to the barbed ends of actin filaments, preventing further polymerization and thereby stabilizing the cytoskeletal architecture. Research has indicated that CAPZA1 is involved in various cellular processes, including cell migration, adherence, and signal transduction, which makes it a key player in development and disease. Dysregulation of CAPZA1 has been associated with several pathological conditions, including cancer metastasis and neurodegenerative diseases. Therefore, the study of CAPZA1 encompasses not only its physiological roles but also its potential as a therapeutic target. Recombinant CAPZA1 proteins have been produced to facilitate detailed investigations into their structural properties and interactions with actin, providing insights into the mechanisms of cytoskeletal regulation. Understanding the functional implications of CAPZA1 in cellular processes may yield significant advances in the fields of cell biology and therapeutic interventions for conditions linked to cytoskeletal abnormalities. Research efforts continue to explore the pathways influenced by CAPZA1, with hopes of elucidating its role in normal physiology and pathology, ultimately offering avenues for targeted treatment strategies.

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