Analytical Data
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基因名
REC8L1
- Application
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别名
Meiotic recombination protein REC8 homolog. Cohesin Rec8p
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O95072
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表达区间
1-547 aa
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氨基酸序列
MFYYPNVLQRHTGCFATIWLAATRGSRLVKREYLRVNVVKTCEEILNYVLVRVQPPQPGLPRPRFSLYLSAQLQIGVIRVYSQQCQYLVEDIQHILERLHRAQLQIRIDMETELPSLLLPNHLAMMETLEDAPDPFFGMMSVDPRLPSPFDIPQIRHLLEAAIPERVEEIPPEVPTEPREPERIPVTVLPPEAITILEAEPIRMLEIEGERELPEVSRRELDLLIAEEEEAILLEIPRLPPPAPAEVEGIGEALGPEELRLTGWEPGALLMEVTPPEELRLPAPPSPERRPPVPPPPRRRRRRRLLFWDKETQISPEKFQEQLQTRAHCWECPMVQPPERTIRGPAELFRTPTLSGWLPPELLGLWTHCAQPPPKALRRELPEEAAAEEERRKIEVPSEIEVPREALEPSFPLMVSLEISLEAAEEEKSRISLIPPEERWAWPEVEAPEAPALPVVPELPEVPMEMPLVLPPELELLSLEAVHRAVALELQANREPDFSSLVSPLSPRRMAARVFYLLLVLSAQQILHVKQEKPYGRLLIQPGPRFH
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分子量
89.1 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
REC8L1 is a member of the REC8 family of cohesin proteins, critical for maintaining sister chromatid cohesion during meiosis and mitosis. The study of REC8L1 has gained significant attention due to its essential role in chromosome segregation and the regulation of gene expression. Research indicates that REC8L1 is involved in the establishment and maintenance of chromatin structures, thus influencing cellular processes such as DNA repair and replication. Dysregulation of REC8L1 has been associated with various developmental disorders and cancers, highlighting its potential as a therapeutic target. Understanding the molecular mechanisms underlying REC8L1's function could provide insights into chromosome dynamics and inform strategies for treating diseases linked to genomic instability. Recent advances in protein engineering have allowed for the successful expression and purification of recombinant REC8L1, facilitating detailed structural and functional analyses. Such studies aim to elucidate the protein's interactions with other cellular components and its contribution to chromosomal behavior during cell division, fostering a deeper appreciation of its role in cellular physiology and disease. Scientists are increasingly employing techniques like CRISPR gene editing and advanced imaging to explore the dynamics of REC8L1 in live cells, aiming to provide a comprehensive understanding of its biological significance.
