Analytical Data
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Gene name
RCL1
- Application
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Alternative Names
RNA 3'-terminal phosphate cyclase-like protein
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9Y2P8
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Expression Region
1-373 aa
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AA Sequence
MATQAYSLSYAGCNFLRQRLVLSTLSGRPVKIRKIRARDDNPGLRDFEASFIRLLDKITNGSRIEINQTGTTLYYQPGLLYGGSVEHDCSVLRGIGYYLESLLCLAPFMKHPLKIVLRGVTNDQVDPSVDVLKATALPLLKQFGIDGESFELNIVRRGMPPGGGGEVVFSCPVRKVLKPIQLTDPGKIKRIRGMAYSVRVSPQMANRIVDSARSILNKFIPDIYIYTDHMKGVNSGKSPGFGLSLVAETTSGTFLSAELASNPQGQGAAVLPEDLGRNCARLLLEEIYRGGCVDSTNQSLALLLMTLGQQDVSKVLLGPLSPYTIEFLRHLKSFFQIMFKIETKPCGEELKGGDKVLMTCVGIGFSNLSKTLK
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Molecular Weight
67.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RCL1 (recombination repair protein) is a crucial component in the regulation of mRNA splicing and the maintenance of genome stability. Recent studies have revealed its multifaceted roles in various cellular processes, including DNA repair and the regulation of gene expression. RCL1 is known to associate with the spliceosome, the molecular machine responsible for excising introns from pre-mRNA, thereby playing a vital role in the maturation of mRNA. Dysregulation of RCL1 has been linked to several diseases, including cancer, as it can affect the splicing of genes involved in cell proliferation and apoptosis. Additionally, RCL1 has been implicated in the response to cellular stress, highlighting its importance in maintaining cellular homeostasis. Understanding RCL1's structure and function is essential for elucidating its role in gene regulation and its potential as a therapeutic target in diseases associated with splicing defects. Research into RCL1 recombinantly expressed protein can provide insights into its interactions and regulatory mechanisms, as well as its potential implications in therapeutic strategies aimed at correcting splicing anomalies linked to various pathologies.
