Analytical Data
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Gene name
RBPJ
- Application
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Alternative Names
Recombining binding protein suppressor of hairless. J kappa-recombination signal-binding protein. RBP-J kappa
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P31266
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Expression Region
1-487 aa
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AA Sequence
MAPVVTGKFGERPPPKRLTREAMRNYLKERGDQTVLILHAKVAQKSYGNEKRFFCPPPCVYLMGSGWKKKKEQMERDGCSEQESQPCAFIGIGNSDQEMQQLNLEGKNYCTAKTLYISDSDKRKHFMLSVKMFYGNSDDIGVFLSKRIKVISKPSKKKQSLKNADLCIASGTKVALFNRLRSQTVSTRYLHVEGGNFHASSQQWGAFFIHLLDDDESEGEEFTVRDGYIHYGQTVKLVCSVTGMALPRLIIRKVDKQTALLDADDPVSQLHKCAFYLKDTERMYLCLSQERIIQFQATPCPKEPNKEMINDGASWTIISTDKAEYTFYEGMGPVLAPVTPVPVVESLQLNGGGDVAMLELTGQNFTPNLRVWFGDVEAETMYRCGESMLCVVPDISAFREGWRWVRQPVQVPVTLVRNDGIIYSTSLTFTYTPEPGPRPHCSAAGAILRANSSQVPPNESNTNSEGSYTNASTNSTSVTSSTATVVS
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Molecular Weight
80.7 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RBPJ (Recombination Signal Binding Protein for Immunoglobulin Kappa J Region) is a crucial transcription factor that plays a significant role in the Notch signaling pathway, a fundamental mechanism governing cell fate determination, differentiation, and development in various biological contexts. Initially identified in the context of immunology, RBPJ is essential for the regulation of immune cell development by facilitating the transcription of Notch target genes. Abnormalities in RBPJ expression or function have been implicated in several pathological conditions, including cancers, where dysregulation of the Notch pathway contributes to tumorigenesis and cancer progression. Recent studies have explored the therapeutic potential of targeting RBPJ in cancer treatment, emphasizing its role in the proliferation and survival of cancer stem cells. Furthermore, RBPJ is also involved in neurogenesis and has been linked to neurodegenerative diseases, making it a dual focus of research in both oncology and neurology. Understanding the molecular mechanisms underlying RBPJ's function and its intricate interactions within various cellular pathways is crucial for developing innovative therapeutic strategies to manipulate its activity for disease intervention. This research is especially pertinent in the face of increasing interest in the Notch pathway as a target for drug development, given its broad implications in physiology and disease.
