Analytical Data
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Gene name
AMA-1
- Application
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Alternative Names
AMA-1；Apical membrane antigen 1
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P50492
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Expression Region
194-546aa
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AA Sequence
TLDHMRDFYKNNEYVKNLDELTLCSRHAGNMNPDNDKNSNYKYPAVYDYNDKKCHILYIAAQENNGPRYCNKDESKRNSMFCFRPAKDKSFQNYTYLSKNVVDNWEKVCPRKNLENAKFGLWVDGNCEDIPHVNEFSANDLFECNKLVFELSASDQPKQYEQHLTDYEKIKEGFKNKNASMIKSAFLPTGAFKADRYKSRGKGYNWGNYNRKTQKCEIFNVKPTCLINNSSYIATTALSHPNEVEHNFPCSLYKDEIKKEIERESKRIKLNDNDDEGNKKIIAPRISISDDIDSLKCPCDPEIVSNSTCNFFVCKCVEKRAEVTSNNEVVVKEEYKDEYADIPEHKPTYDKMK
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Molecular Weight
45.2 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
AMA-1 (apical membrane antigen 1) is a pivotal protein in the life cycle of Plasmodium species, the parasites responsible for malaria. This protein is expressed on the surface of merozoites, the invasive form of the parasite that infects red blood cells. Given its critical role in host cell invasion, AMA-1 has garnered significant attention as a potential target for malaria vaccine development. Research has shown that AMA-1 is highly conserved across different Plasmodium species, which enhances its viability as a universal vaccine candidate. Additionally, antibodies generated against AMA-1 have demonstrated the ability to inhibit parasite invasion in vitro, suggesting a promising avenue for immunization strategies. However, challenges remain in terms of the protein's expression, purification, and immunogenicity. Advances in recombinant DNA technology have facilitated the generation of AMA-1 recombinant proteins, allowing for detailed studies of their structure and function. Understanding the immunological responses to these recombinant proteins is crucial for optimizing vaccine formulations. Recent studies have also explored the potential of AMA-1 in combination with other antigens to improve vaccine efficacy. Overall, the investigation of AMA-1 recombinant protein serves as a cornerstone for malaria vaccine research, aiming to curb the global burden of this disease.
