Analytical Data
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基因名
CD34
- Application
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别名
CD34；Hematopoietic progenitor cell antigen CD34
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P28906
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表达区间
33-290aa
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氨基酸序列
LDNNGTATPE LPTQGTFSNV STNVSYQETT TPSTLGSTSL HPVSQHGNEA TTNITETTVK FTSTSVITSV YGNTNSSVQS QTSVISTVFT TPANVSTPET TLKPSLSPGN VSDLSTTSTS LATSPTKPYT SSSPILSDIK AEIKCSGIRE VKLTQGICLE QNKTSSCAEF KKDRGEGLAR VLCGEEQADA DAGAQVCSLL LAQSEVRPQC LLLVLANRTE ISSKLQLMKK HQSDLKKLGI LDFTEQDVAS HQSYSQKT
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分子量
27 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CD34 is a glycoprotein expressed on the surface of hematopoietic stem and progenitor cells, playing a vital role in cell adhesion, migration, and differentiation. It serves as a widely recognized marker for identifying and isolating these stem cells in research and clinical applications, particularly in hematology and oncology. The recombinant production of CD34 protein has gained significant interest due to its potential therapeutic implications and applications in regenerative medicine. Understanding the structure and function of CD34 at the molecular level is crucial for developing targeted therapies for various blood disorders and improving stem cell transplantation techniques. Researchers have been exploring different expression systems and purification methods to optimize the yield and activity of recombinant CD34, aiming to study its interactions with specific ligands and signaling pathways. This knowledge can enhance our understanding of hematopoietic cell biology and facilitate advancements in tissue engineering and cell-based therapies. Consequently, the investigation of CD34 recombinant protein is a key focus in the fields of stem cell research and regenerative medicine, promising new avenues for treating a range of conditions related to the hematopoietic system.
