Analytical Data
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基因名
PPDK
- Application
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别名
PPDK；Pyruvate. phosphate dikinase 1. chloroplastic
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种属
Entamoeba
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P37213
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表达区间
1-342aa
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氨基酸序列
MQRVYAFEDGDGTNKKLLGGKGAGLCTMTKIGLPVPQGFVITTEMCKQFIANGNKMPEGLMEEVKKEYQLVEKKSGKVFGGEENPLLVSVRSGAAMSMPGMMDTILNLGLNDKTVVALAKLTNNERFAYDSYRRFVSLFGKIALNACDEVYDKTLENKKVEKGVKLDTELDANDMKELAQVFIKKTEEFTKQPFPVDPYAQLEFAICAVFRSWMGKRAVDYRREFKITPEQADGTAVSVVSMVYGNMGNDSATGVCFTRDPGTGENMFFGEYLKNAQGEDVVAGIRTPQIISKMAEDRDLPGCYEQLLDIRKKLEGYFHEVQDFEFTIERKKLYMLQTRNGK
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分子量
54.4 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PPDK (pyruvate phosphate dikinase) is a crucial enzyme involved in the metabolic pathways of various organisms, particularly in plants and some bacteria. It plays a significant role in regulating energy metabolism by catalyzing the conversion of pyruvate and phosphate into phosphoenolpyruvate (PEP), an essential substrate for numerous biosynthetic processes. Research on PPDK is vital due to its potential applications in agriculture and biotechnology, particularly in enhancing crop yield and stress resilience. Understanding its structure and function can lead to the development of genetically engineered plants with improved photosynthetic efficiency and resource utilization. Furthermore, PPDK's unique properties, such as its allosteric regulation and its role in C4 and CAM (Crassulacean Acid Metabolism) photosynthesis, make it a focal point for investigating carbon fixation mechanisms and optimizing biomass production. Advances in recombinant protein technology have facilitated detailed studies of PPDK, allowing researchers to explore its kinetics, molecular interactions, and functional dynamics more comprehensively. This has implications not just for basic plant biology but also for addressing global challenges like food security and environmental sustainability through innovative agricultural practices.
