Analytical Data
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Gene name
RASGRF1
- Application
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Alternative Names
CDC25 ; CDC25L; GNRP; GRF1 ; GRF55 ; Guanine nucleotide exchange factor ; Guanine nucleotide releasing factor 55 kD; Guanine nucleotide releasing protein ; guanine nucleotide-releasing factor 1; Guanine nucleotide-releasing protein; H GRF55; HGRF55; PP13187 ; Ras protein specific guanine nucleotide releasing factor 1 ; Ras specific guanine nucleotide releasing factor; Ras specific guanine nucleotide releasing factor CDC25 homolog; Ras specific nucleotide exchange factor CDC25; Ras-GRF1; Ras-specific guanine nucleotide-releasing factor 1; Ras-specific nucleotide exchange factor CDC25; RASGRF1; RGRF1_HUMAN
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q13972
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Expression Region
1-110 aa
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AA Sequence
MQKGIRLNDGHVASLGLLARKDGTRKGYLSKRSSDNTKWQTKWFALLQNLLFYFESDSSSRPSGLYLLEGCVCDRAPSPKPALSAKEPLEKQHYFTVNFSHENQKALELR
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Molecular Weight
37.84 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
RASGRF1, a member of the RAS gene family, plays a crucial role as a guanine nucleotide exchange factor (GEF) that activates RAS proteins, which are pivotal in various signaling pathways governing cell proliferation, differentiation, and survival. Alterations in RASGRF1 expression have been associated with several human diseases, including cancer, where its dysregulation can lead to aberrant cell signaling and tumorigenesis. The study of RASGRF1 as a recombinant protein has gained traction due to its potential therapeutic implications; understanding its structure-function relationships and interaction with RAS proteins can provide insights into novel cancer treatments. Furthermore, the investigation of RASGRF1's role in neuronal development and function highlights its significance in neurobiology, as its expression levels are dynamically regulated in the brain. Researchers have been utilizing recombinant DNA technology to produce and characterize RASGRF1, aiming to elucidate its molecular mechanisms and how it influences various cellular processes. Overall, the exploration of RASGRF1 as a recombinant protein opens avenues for targeted therapies and provides a deeper understanding of its contributions to human health and disease.
