Analytical Data
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Gene name
ureA
- Application
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Alternative Names
ureA；HUT2；UT2；Urea transporter 2
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O13465
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Expression Region
1-833aa
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AA Sequence
MHLLPRETDKLILTTLGTLAQRRLARGLILNRAETIALISSQLQEFVRDGRHSVAELMDLGKKMLGRRHVRKGVPESIHTIQVEGTFPDGVFLVTVDDPISSDDGDLNNAFYGSFLPIPSADVFPAAPEPADTLLGALICRKETVKINASRRRFRLEVKNAGDRPVQVGSHYHFLETNPALIFDRLLSYGYHLDIPAGTAVRFEPGEKKTVTMVEFGGKKIFHGGSGLGNGSFDENLRETKVKEMVEKGGFGHKEQEKIEEGPVTEMNREVYASMFGPTTGDKIKLADMDLWIEVEKDYTVYGDECKFGGGKVIRDGGGQASGRHDHEVLDLVITNALIVDWTGIYKADIGVKNGIIVGIGKAGNPDMMDGVTDGMIVGSSTEVISGEKLITTAGALDVHVHYISPQLMTEALASGITTVIGGGTGPADGSNATTCTSSSFYMQNMIKATDTIPLNFGFTGKGSDSGTNAMRDIIEAGACGLKVHEDWGATPEVIDRALSMADEYDVQINLHSDTLNESGYVESTLAAIKGRTIHSYHTEGAGGGHAPDIIVVCEYENVLPSSTNPTRPYAVNTLDEHLDMLMICHGLDKSIPEDIAFADSRIRSETVAAEDVLQDTGAISMISSDCQAMGRIGEVVTRTWRTAAKMKQFRGPLEGDEPTRDNNRVKRYVAKYTINPAITHGMSHLIGQVAVGCLADLVFWTAESFGARPEMILKGGVIAWAAVGDANASIPTVQPVLGRPMWGSQPEAAALNSIVWVSQASLDKDLVKRFNIKKRAEAVKNCRSIGKKDMKWNDTMPKMTVDPETYDVRADGVLCDVPPADKLPLTRRYFVY
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Molecular Weight
90.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Urease, an enzyme that catalyzes the hydrolysis of urea into ammonia and carbon dioxide, plays a crucial role in nitrogen metabolism across various organisms, including bacteria, plants, and some fungi. The urease enzyme from *Helicobacter pylori*, a bacterium linked to gastric ulcers and cancer, has garnered significant interest due to its unique properties and potential applications in biotechnology and medicine. Research into urease has revealed its complex multi-subunit structure, its dependence on nickel ions for activity, and its role in the survival of *H. pylori* in acidic environments. To further investigate the enzyme's function and facilitate the development of urease inhibitors as therapeutic agents, scientists have turned to recombinant DNA technology to produce urease subunits in heterologous systems, such as *Escherichia coli*. By expressing the ureA gene, which encodes one of the enzyme’s subunits, researchers aim to explore the enzyme's characteristics, including its activity, stability, and interaction with other proteins. These studies contribute to a deeper understanding of urease's mechanisms and its potential implications in agriculture, environmental remediation, and clinical settings, offering opportunities for innovative solutions in managing nitrogen levels and treating related diseases.
