Analytical Data
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Gene name
degS
- Application
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Alternative Names
degS；C14orf66；Sphingolipid delta(4)-desaturase/C4-monooxygenase DES2
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P0AEE3
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Expression Region
28-355aa
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AA Sequence
SLN PLSTPQFDST DETPASYNLA VRRAAPAVVN VYNRGLNTNS HNQLEIRTLG SGVIMDQRGY IITNKHVIND ADQIIVALQD GRVFEALLVG SDSLTDLAVL KINATGGLPT IPINARRVPH IGDVVLAIGN PYNLGQTITQ GIISATGRIG LNPTGRQNFL QTDASINHGN SGGALVNSLG ELMGINTLSF DKSNDGETPE GIGFAIPFQL ATKIMDKLIR DGRVIRGYIG IGGREIAPLH AQGGGIDQLQ GIVVNEVSPD GPAANAGIQV NDLIISVDNK PAISALETMD QVAEIRPGSV IPVVVMRDDK QLTLQVTIQE YPATN
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Molecular Weight
37.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
DegS is a periplasmic protease and a serine protease that plays a critical role in bacterial stress responses, particularly in the regulation of the Cpx envelope stress response system in Escherichia coli. Under stress conditions, such as misfolded proteins in the periplasm, DegS acts as a sensor that activates the Cpx regulon, leading to enhanced cellular adaptation. This protease recognizes specific substrates and can influence protein stability and function, impacting bacterial viability under environmental stressors. The study of DegS recombinant protein is significant for understanding the molecular mechanisms underlying bacterial stress adaptation and pathogenesis. By investigating the structure and function of DegS, researchers aim to elucidate its role in stress response pathways, which may uncover novel targets for antimicrobial development. Furthermore, DegS's dual role as a protease and a signaling molecule highlights its potential as a therapeutic target in combating bacterial infections and understanding resistance mechanisms. Thus, research into DegS and its recombinant proteins not only enhances our fundamental knowledge of bacterial physiology but also paves the way for innovative strategies in antibiotic research and drug design.
