Analytical Data
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基因名
alaC
- Application
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别名
alaC；yfdZ；Glutamate-pyruvate aminotransferase AlaC
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种属
E.coli
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P77434
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表达区间
1-412aa
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氨基酸序列
MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMGNPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAIVTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNELERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVYDGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYGTFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKIPEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKAMFRADGLLPASSKHIHENAE
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分子量
62.2 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
AlaC is a pivotal protein in various biological processes, particularly in the regulation of amino acid metabolism and cellular signaling. Its significance has garnered attention in the fields of microbiology and biochemistry due to its role in the biosynthesis of amino acids and its potential implications in metabolic engineering. Research on AlaC often focuses on its structure-function relationship, revealing the intricate mechanisms by which it interacts with substrates and regulates metabolic pathways. The recombinant expression of AlaC provides invaluable insights into its functionality, facilitating detailed studies on enzyme kinetics, inhibition, and substrate specificity. Additionally, understanding AlaC’s role in different organisms can illuminate evolutionary adaptations and regulatory mechanisms at play in diverse environments. The application of recombinant AlaC in synthetic biology and biotechnology holds promise for the development of novel metabolic pathways, which could enhance the production of valuable biomolecules. As researchers continue to explore AlaC’s structure and function, the protein stands as a vital target for biotechnological innovations and therapeutic interventions, making it an essential subject of study in contemporary biochemical research.
