Analytical Data
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基因名
ureB
- Application
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别名
ureB；KIAA0312；KIAA1578；UREB1；E3 ubiquitin-Protein ligase HUWE1
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种属
E.coli
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P69996
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表达区间
1-569aa
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氨基酸序列
MKKISRKEYVSMYGPTTGDKVRLGDTDLIAEVEHDYTIYGEELKFGGGKTLREGMSQSNNPSKEELDLIITNALIVDYTGIYKADIGIKDGKIAGIGKGGNKDMQDGVKNNLSVGPATEALAGEGLIVTAGGIDTHIHFISPQQIPTAFASGVTTMIGGGTGPADGTNATTITPGRRNLKWMLRAAEEYSMNLGFLAKGNASNDASLADQIEAGAIGFKIHEDWGTTPSAINHALDVADKYDVQVAIHTDTLNEAGCVEDTMAAIAGRTMHTFHTEGAGGGHAPDIIKVAGEHNILPASTNPTIPFTVNTEAEHMDMLMVCHHLDKSIKEDVQFADSRIRPQTIAAEDTLHDMGIFSITSSDSQAMGRVGEVITRTWQTADKNKKEFGRLKEEKGDNDNFRIKRYLSKYTINPAIAHGISEYVGSVEVGKVADLVLWSPAFFGVKPNMIIKGGFIALSQMGDANASIPTPQPVYYREMFAHHGKAKYDANITFVSQAAYDKGIKEELGLERQVLPVKNCRNITKKDMQFNDTTAHIEVNPETYHVFVDGKEVTSKPANKVSLAQLFSIF
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分子量
61.7 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
UreB is a significant component of the urease enzyme complex, which catalyzes the hydrolysis of urea into ammonia and carbon dioxide, playing a crucial role in nitrogen metabolism in various organisms, including plants and microbes. The study of UreB is particularly important due to its potential applications in agricultural biotechnology and environmental management, as urease activity is linked to nitrogen cycling in soils. Additionally, UreB has been a focal point in medical research due to the role of urease in the pathogenesis of certain infections, such as those caused by Helicobacter pylori, which is associated with stomach ulcers and cancer. Recombinant UreB proteins can provide valuable insights into the enzyme's structure and function, enabling the exploration of its kinetic properties and interactions with other proteins. Furthermore, the ability to produce UreB through recombinant DNA technology allows for the development of specific inhibitors, which can act as therapeutic agents by mitigating the harmful effects of excessive urease activity. Overall, research on recombinant UreB not only enhances our understanding of fundamental biological processes but also opens pathways for innovative solutions in health and agriculture.
