Analytical Data
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Gene name
ydhF
- Application
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Alternative Names
ydhF；Oxidoreductase YdhF
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P76187
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Expression Region
1-298aa
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AA Sequence
MVQRITIAPQ GPEFSRFVMG YWRLMDWNMS ARQLVSFIEE HLDLGVTTVD HADIYGGYQC EAAFGEALKL APHLRERMEI VSKCGIATTA REENVIGHYI TDRDHIIKSA EQSLINLATD HLDLLLIHRP DPLMDADEVA DAFKHLHQSG KVRHFGVSNF TPAQFALLQS RLPFTLATNQ VEISPVHQPL LLDGTLDQLQ QLRVRPMAWS CLGGGRLFND DYFQPLRDEL AVVAEELNAG SIEQVVYAWV LRLPSQPLPI IGSGKIERVR AAVEAETLKM TRQQWFRIRK AALGYDVP
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Molecular Weight
33.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The study of the ydhF recombinant protein has gained significant attention due to its potential roles in bacterial physiology and metabolism. ydhF, often considered a hypothetical gene, encodes a protein involved in various cellular processes, including stress response and regulation of metabolic pathways. Research has indicated that ydhF may play a crucial role in the survival of bacteria under adverse conditions, such as oxidative stress and nutrient limitation. Moreover, understanding the structure and function of the ydhF protein can provide insights into its mechanism of action and interactions with other cellular components. Investigating ydhF also holds promise for biotechnological applications, such as the development of novel antimicrobial agents or metabolic engineering strategies in recombinant DNA technology. Given that many bacterial pathogens possess ydhF homologs, this protein could serve as a promising target for therapeutic intervention, potentially leading to new strategies for combating bacterial infections. Consequently, characterizing ydhF and its recombinant forms could contribute significantly to advancing our knowledge of microbial pathogenesis and developing effective treatment modalities.
