Analytical Data
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基因名
NET1
- Application
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别名
NET1；NTN1L；Netrin-1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q7Z628
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表达区间
1-542aa
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氨基酸序列
MVAHDETGGLLPIKRTIRVLDVNNQSFREQEEPSNKRVRPLARVTSLANLISPVRNGAVRRFGQTIQSFTLRGDHRSPASAQKFSSRSTVPTPAKRRSSALWSEMLDITMKESLTTREIRRQEAIYEMSRGEQDLIEDLKLARKAYHDPMLKLSIMSEEELTHIFGDLDSYIPLHEDLLTRIGEATKPDGTVEQIGHILVSWLPRLNAYRGYCSNQLAAKALLDQKKQDPRVQDFLQRCLESPFSRKLDLWSFLDIPRSRLVKYPLLLKEILKHTPKEHPDVQLLEDAILIIQGVLSDINLKKGESECQYYIDKLEYLDEKQRDPRIEASKVLLCHGELRSKSGHKLYIFLFQDILVLTRPVTRNERHSYQVYRQPIPVQELVLEDLQDGDVRMGGSFRGAFSNSEKAKNIFRIRFHDPSPAQSHTLQANDVFHKQQWFNCIRAAIAPFQSAGSPPELQGLPELHEECEGNHPSARKLTAQRRASTVSSVTQVEVDENAYRCGSGMQMAEDSKSLKTHQTQPGIRRARDKALSGGKRKETLV
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分子量
77.9kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of NET1 recombinant proteins is rooted in the exploration of their pivotal roles in cell signaling and the regulation of cellular processes. NET1, or neuroepithelial cell transforming gene 1, functions primarily as a guanine nucleotide exchange factor (GEF) for Rho GTPases, influencing various cellular functions such as cytoskeletal organization, cell migration, and division. Abnormal regulation of NET1 has been implicated in several pathologies, including cancer and cardiovascular diseases, making it a crucial target for therapeutic intervention. Researchers have been particularly interested in the potential of NET1 as a biomarker for disease progression and as a potential therapeutic target. The development of recombinant NET1 proteins allows for detailed structural and functional studies that can elucidate its mechanisms of action and interactions with other cellular molecules. By generating these recombinant proteins, scientists can perform assays to better understand NET1's role in physiological and pathological conditions, paving the way for potential clinical applications in diagnostics and treatment strategies. This research not only contributes to the fundamental understanding of cellular signaling but also holds promise for the development of novel therapeutic approaches targeting NET1-related pathways.
