Analytical Data
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Gene name
NET1
- Application
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Alternative Names
NET1；NTN1L；Netrin-1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q7Z628
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Expression Region
1-542aa
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AA Sequence
MVAHDETGGLLPIKRTIRVLDVNNQSFREQEEPSNKRVRPLARVTSLANLISPVRNGAVRRFGQTIQSFTLRGDHRSPASAQKFSSRSTVPTPAKRRSSALWSEMLDITMKESLTTREIRRQEAIYEMSRGEQDLIEDLKLARKAYHDPMLKLSIMSEEELTHIFGDLDSYIPLHEDLLTRIGEATKPDGTVEQIGHILVSWLPRLNAYRGYCSNQLAAKALLDQKKQDPRVQDFLQRCLESPFSRKLDLWSFLDIPRSRLVKYPLLLKEILKHTPKEHPDVQLLEDAILIIQGVLSDINLKKGESECQYYIDKLEYLDEKQRDPRIEASKVLLCHGELRSKSGHKLYIFLFQDILVLTRPVTRNERHSYQVYRQPIPVQELVLEDLQDGDVRMGGSFRGAFSNSEKAKNIFRIRFHDPSPAQSHTLQANDVFHKQQWFNCIRAAIAPFQSAGSPPELQGLPELHEECEGNHPSARKLTAQRRASTVSSVTQVEVDENAYRCGSGMQMAEDSKSLKTHQTQPGIRRARDKALSGGKRKETLV
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Molecular Weight
77.9kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The study of NET1 recombinant proteins is rooted in the exploration of their pivotal roles in cell signaling and the regulation of cellular processes. NET1, or neuroepithelial cell transforming gene 1, functions primarily as a guanine nucleotide exchange factor (GEF) for Rho GTPases, influencing various cellular functions such as cytoskeletal organization, cell migration, and division. Abnormal regulation of NET1 has been implicated in several pathologies, including cancer and cardiovascular diseases, making it a crucial target for therapeutic intervention. Researchers have been particularly interested in the potential of NET1 as a biomarker for disease progression and as a potential therapeutic target. The development of recombinant NET1 proteins allows for detailed structural and functional studies that can elucidate its mechanisms of action and interactions with other cellular molecules. By generating these recombinant proteins, scientists can perform assays to better understand NET1's role in physiological and pathological conditions, paving the way for potential clinical applications in diagnostics and treatment strategies. This research not only contributes to the fundamental understanding of cellular signaling but also holds promise for the development of novel therapeutic approaches targeting NET1-related pathways.
