Analytical Data
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Gene name
RAB7
- Application
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Alternative Names
CMT2B ; PRO2706; PSN; RAB7; member RAS oncogene family; RAB7A; RAB7A; member RAS oncogene family; RAB7A_HUMAN; Ras associated protein RAB7 ; Ras related protein Rab7; Ras related protein Rab7a; Ras-related protein Rab-7a
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P51149
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Expression Region
1-207 aa
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AA Sequence
MTSRKKVLLK VIILGDSGVG KTSLMNQYVN KKFSNQYKAT IGADFLTKEV MVDDRLVTMQ IWDTAGQERF QSLGVAFYRG ADCCVLVFDV TAPNTFKTLD SWRDEFLIQA SPRDPENFPF VVLGNKIDLE NRQVATKRAQ AWCYSKNNIP YFETSAKEAI NVEQAFQTIA RNALKQETEV ELYNEFPEPI KLDKNDRAKA SAESCSC
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Molecular Weight
23.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Rab7 is a member of the Rab GTPase family, which plays a crucial role in regulating intracellular vesicle trafficking, particularly in the late endosomal and lysosomal pathways. This small GTPase is essential for the maturation of endosomes, the fusion of late endosomes with lysosomes, and the degradation of cellular components. Research has shown that Rab7 is implicated in various cellular processes, including autophagy, nutrient sensing, and the transport of signaling molecules. Dysregulation of Rab7 has been linked to several diseases, including neurodegenerative disorders, cancer, and infections, making it a significant target for therapeutic interventions. The study of recombinant Rab7 proteins has gained attention for elucidating its functional mechanisms and interactions with other cellular proteins. By expressing and purifying Rab7 in a heterologous system, researchers can investigate its GTPase activity, binding partners, and role in cellular dynamics. Understanding the structural and functional properties of Rab7 promotes insights into its contributions to cellular homeostasis and pathogenesis, paving the way for potential drug discovery and development strategies aimed at modulating Rab7 activity in various disease contexts. Thus, the study of recombinant Rab7 proteins not only enhances our understanding of fundamental cellular biology but also holds promise for clinical applications in treating Rab7-associated diseases.
