Analytical Data
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Gene name
ENO2
- Application
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Alternative Names
ENO2；Gamma-enolase
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Species
Mouse
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P17183
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Expression Region
1-434aa
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AA Sequence
MGSSHHHHHHSSGLVPRGSHMGSMSIEKIWAREILDSRGNPTVEVDLYTA KGLFRAAVPSGASTGIYEALELRDGDKQRYLGKGVLKAVDHINSRIAPAL ISSGISVVEQEKLDNLMLELDGTENKSKFGANAILGVSLAVCKAGAAERD LPLYRHIAQLAGNSDLILPVPAFNVINGGSHAGNKLAMQEFMILPVGAES FRDAMRLGAEVYHTLKGVIKDKYGKDATNVGDEGGFAPNILENSEALELV KEAIDKAGYTEKMVIGMDVAASEFYRDGKYDLDFKSPADPSRYITGDQLG ALYQDFVRNYPVVSIEDPFDQDDWAAWSKFTANVGIQIVGDDLTVTNPKR IERAVEEKACNCLLLKVNQIGSVTEAIQACKLAQENGWGVMVSHRSGETE DTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLMRIEEELGDEARFAGHN FRNPSVL
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Molecular Weight
50 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ENO2, or enolase 2, is a glycolytic enzyme that plays a crucial role in cellular metabolism and energy production. It is primarily expressed in the brain and is considered a neuron-specific enolase, making it a potential biomarker for neurological diseases. Research on ENO2 has gained momentum due to its involvement in various pathological conditions, including neurodegenerative diseases, ischemic brain injury, and cancers. The protein exists in several isoforms, with ENO2 being distinctively associated with neuron function and development. Its overexpression has been linked to tumorigenesis, while its levels can serve as an indicator of neuronal damage, making it a target of intense study in both cancer and neuroscience fields. Additionally, ENO2 is being explored for its potential role in mediating neuronal signaling and plasticity, which are fundamental to cognitive processes. The recombinant production of ENO2 allows for detailed investigation into its structure-function relationships and interactions with other biomolecules, fostering insights that could lead to novel therapeutic strategies. As a versatile research tool, the availability of ENO2 as a recombinant protein supports advances in diagnostic applications and therapeutic interventions, highlighting its significance in both basic research and clinical settings. The ongoing studies aim not only to elucidate the biological functions of ENO2 but also to harness its potential for innovative approaches in treating neurodegenerative disorders and cancers, emphasizing the importance of this enzyme in health and disease.
