Analytical Data
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基因名
ALDH7A1
- Application
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别名
ALDH7A1；ATQ1；Alpha-aminoadipic semialdehyde dehydrogenase
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P49419
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表达区间
27-539aa
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氨基酸序列
AFMS TLLINQPQYA WLKELGLREE NEGVYNGSWG GRGEVITTYC PANNEPIARV RQASVADYEE TVKKAREAWK IWADIPAPKR GEIVRQIGDA LREKIQVLGS LVSLEMGKIL VEGVGEVQEY VDICDYAVGL SRMIGGPILP SERSGHALIE QWNPVGLVGI ITAFNFPVAV YGWNNAIAMI CGNVCLWKGA PTTSLISVAV TKIIAKVLED NKLPGAICSL TCGGADIGTA MAKDERVNLL SFTGSTQVGK QVGLMVQERF GRSLLELGGN NAIIAFEDAD LSLVVPSALF AAVGTAGQRC TTARRLFIHE SIHDEVVNRL KKAYAQIRVG NPWDPNVLYG PLHTKQAVSM FLGAVEEAKK EGGTVVYGGK VMDRPGNYVE PTIVTGLGHD ASIAHTETFA PILYVFKFKN EEEVFAWNNE VKQGLSSSIF TKDLGRIFRW LGPKGSDCGI VNVNIPTSGA EIGGAFGGEK HTGGGRESGS DAWKQYMRRS TCTINYSKDL PLAQGIKFQ
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ALDH7A1, or Aldehyde Dehydrogenase 7 Family Member A1, is a crucial enzyme in the detoxification of endogenous aldehydes and plays a significant role in the metabolism of neurotransmitters and amino acids. Mutations in the ALDH7A1 gene are linked to pyridoxine-dependent epilepsy (PDE), a rare but severe neurological disorder that presents in infancy with seizures unresponsive to conventional treatments. The characterization and production of recombinant ALDH7A1 protein are essential for understanding its enzymatic functions, elucidating the mechanisms by which its deficiency leads to pathology, and developing potential therapeutic strategies. By generating this recombinant protein, researchers can explore its substrate specificity, inhibition characteristics, and the impact of various mutations associated with PDE. Furthermore, studies utilizing the recombinant enzyme may help in the design of small molecule activators or gene therapy approaches to restore functional activity in affected individuals. The research on ALDH7A1 not only enhances our fundamental understanding of enzyme biology but also has significant implications for the development of targeted treatments for individuals suffering from pyridoxine-dependent epilepsy and related disorders.
